Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-3-1
|
| pubmed:abstractText |
Recent reports revealed that Fas antigen is functionally expressed on human synovial cells and apoptosis can be induced in these cells by anti-Fas antibody. We examined the effect of interleukin 1 beta (IL-1 beta) on Fas antigen-mediated apoptosis on human synovial cells in vitro. Using flowcytometric analysis, IL-1 beta inhibited Fas antigen expression on synovial cells in a dose-dependent fashion. No significant difference of Fas antigen gene expression between IL-1 beta-treated and untreated synovial cells was observed by RT-PCR analysis, suggesting that the inhibitory effect of Fas antigen expression by IL-1 beta is at posttranscriptional level. Apoptosis of synovial cells was easily induced by treatment of these cells with anti-Fas antibody. In contrast, pretreatment of synovial cells with IL-1 beta protected these cells against Fas antigen-mediated apoptosis. The expression of bcl-2 on synovial cells, known to interfere with the apoptotic process mediated by the Fas antigen, was not influenced by IL-1 beta. Our results suggest that IL-1 beta inhibits Fas antigen-mediated apoptosis of synovial cells and may perpetuate the hyperplasia of the synovium in patients with rheumatoid arthritis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
218
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
280-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8573146-Antigens, CD95,
pubmed-meshheading:8573146-Apoptosis,
pubmed-meshheading:8573146-Arthritis, Rheumatoid,
pubmed-meshheading:8573146-Base Sequence,
pubmed-meshheading:8573146-Cells, Cultured,
pubmed-meshheading:8573146-DNA Primers,
pubmed-meshheading:8573146-Flow Cytometry,
pubmed-meshheading:8573146-Gene Expression Regulation,
pubmed-meshheading:8573146-Humans,
pubmed-meshheading:8573146-Interleukin-1,
pubmed-meshheading:8573146-Kinetics,
pubmed-meshheading:8573146-Lymphocytes,
pubmed-meshheading:8573146-Molecular Sequence Data,
pubmed-meshheading:8573146-Osteoarthritis,
pubmed-meshheading:8573146-Polymerase Chain Reaction,
pubmed-meshheading:8573146-Proto-Oncogene Proteins,
pubmed-meshheading:8573146-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:8573146-Recombinant Proteins,
pubmed-meshheading:8573146-Reference Values,
pubmed-meshheading:8573146-Synovial Membrane
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Fas antigen expression on synovial cells was down-regulated by interleukin 1 beta.
|
| pubmed:affiliation |
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|