Linked Life Data

8567893

Source:http://linkedlifedata.com/resource/pubmed/id/8567893

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1996-3-7
pubmed:abstractText
The sensitivities of several plating and broth enrichment methods for the detection of Escherichia coli O157:H7 in (i) bovine fecal samples directly inoculated with E. coli O157:H7, (ii) fecal samples from cattle in herds previously positive for E. coli O157:H7, and (iii) fecal samples from calves shedding E. coli O157:H7 after experimental oral inoculation were compared. Three enrichment protocols and three plating protocols were evaluated with directly inoculated fecal samples. All broth enrichment methods were superior to direct plating when they were combined with subsequent plating on sorbitol-MacConkey with cefixime and tellurite (SMACct). SMACct was the most sensitive plating medium, and the three alternative broth enrichment methods gave similar improvements in sensitivity. Of 351 fecal samples from known positive herds, 24 samples (6.8%) were positive by one or more methods. By the most sensitive plating method, cultures of 10-g samples were slightly more sensitive (19 of 351 [5.4%]) than cotton-tipped swab fecal samples (14 of 351 [4.0%]); however, this difference was not significant. For samples from calves orally inoculated with E. coli O157:H7, separation by immunomagnetic beads was slightly more sensitive (79%) than broth enrichment followed by plating at two dilutions (10(-3) and 10(-4)) (71%); however, this difference was not significant. The combination of overnight enrichment of swab fecal samples (0.1 g) and plating on SMACct at two dilutions (10(-3) and 10(-4)) appears to be a sensitive method for detection in large-scale studies involving hundreds of samples per week.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-1441140, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-1765120, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-1875390, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-2056066, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-2229338, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-2242412, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-2661252, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-3053758, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-3519658, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-6338386, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-8006935, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-8062877, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-8270004, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-8345511, http://linkedlifedata.com/resource/pubmed/commentcorrection/8567893-8432882
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2616-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Sensitivity of bacteriologic culture for detection of Escherichia coli O157:H7 in bovine feces.
pubmed:affiliation
Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USA.
pubmed:publicationType
Journal Article