Linked Life Data

8567846

Source:http://linkedlifedata.com/resource/pubmed/id/8567846

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1996-3-7
pubmed:abstractText
To determine whether variations in spontaneous and induced acrosome reactions are correlated with semen quality, and to identify the inducers of clinical interest, the acrosome reaction, sperm concentration, motility and morphology were recorded in 117 semen samples from patients undergoing an in-vitro fertilization (IVF) attempt. The spontaneous acrosome loss after 24 h incubation in Ménézo B2 medium and after induction by calcium ionophore A23187, progesterone, human follicular fluid, cyclic adenosine 3'-5'-phosphate (cAMP) analogue and phorbol ester (TPA) were measured using the fluorescein isothiocyanate-GB24 antibody. The mean (range) spontaneous acrosome reaction was 3.4 +/- 0.6% (0.0-30.0). Response to the tested inducers was 23.3 +/- 1.6% (0.0-72.0) for calcium ionophore, 5.4 +/- 0.8% (0.0-60.0) for progesterone, 1.0 +/- 0.7% (0.0-24.0) for human follicular fluid, 2.6 +/- 0.7% (0.0-39.0) for the cAMP analogue and 2.3 +/- 0.7% (0.0-31.0) for TPA. The response to calcium ionophore was correlated significantly to sperm concentration, motility and morphology, while the responses to progesterone and TPA were correlated significantly to motility and acrosome morphology. The responses to other inducers were not linked to classic semen parameters. When studying acrosome reaction as a function of IVF results, the responses to calcium ionophore and TPA were discriminant. The results of this study show that the spontaneous acrosome loss and the responses to acrosome reaction inducers are highly variable and partially linked to semen quality. The responses to calcium ionophore and TPA could be of interest in predicting the fertilizing ability in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2085-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Variations in spontaneous and induced acrosome reaction: correlations with semen parameters and in-vitro fertilization results.
pubmed:affiliation
Laboratoire de Fécondation In Vitro, CHU La Grave, Toulouse, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't