| pubmed-article:8567116 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8567116 | lifeskim:mentions | umls-concept:C0014257 | lld:lifeskim |
| pubmed-article:8567116 | lifeskim:mentions | umls-concept:C0001511 | lld:lifeskim |
| pubmed-article:8567116 | lifeskim:mentions | umls-concept:C1513095 | lld:lifeskim |
| pubmed-article:8567116 | lifeskim:mentions | umls-concept:C0806140 | lld:lifeskim |
| pubmed-article:8567116 | lifeskim:mentions | umls-concept:C0348080 | lld:lifeskim |
| pubmed-article:8567116 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8567116 | pubmed:dateCreated | 1996-3-1 | lld:pubmed |
| pubmed-article:8567116 | pubmed:abstractText | Lu-ECAM-1 is a lung-derived, venular endothelial cell adhesion molecule. It promotes the selective adhesion of lung-metastatic B16-F10 melanoma cells to endothelium under static conditions and mediates colonization of the lungs by the same tumor cells. To test whether Lu-ECAM-1 by itself is sufficient to cause vascular arrest of B16-F10 cells, we measured here under conditions of flow tumor cell adhesion to endothelia that express different amounts of Lu-ECAM-1 on their surfaces. At physiological shear stresses, adhesion of B16-F10 melanoma cells to endothelia correlates positively with the amount of Lu-ECAM-1 expression on the endothelial cell surface and inversely with the level of the applied shear stress. Tumor cell trajectories are biphasic; i.e., B16-F10 melanoma cells initially move along the endothelial surface with a velocity similar to the theoretical velocity, then arrest within a fraction of a second. Arrest is permanent for most B16-F10 melanoma cells at all shear stresses tested. Tumor cells never engaged in a rolling motion prior to arrest. Masking of the Lu-ECAM-1 ligand on the surface of B16-F10 melanoma cells with soluble Lu-ECAM-1 impedes arrest of tumor cells on the surface of the test endothelium. Purified Lu-ECAM-1 also mediates B16-F10 arrest, but arrest is mostly transient at shear stresses of 0.59 dynes/cm2 and higher, implying adhesion by single receptor/ligand bonds. Our data suggest that Lu-ECAM-1 plays a critical role in the recognition and initial arrest of murine melanoma cells in lung venules. | lld:pubmed |
| pubmed-article:8567116 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8567116 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8567116 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8567116 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8567116 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8567116 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8567116 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8567116 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8567116 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8567116 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8567116 | pubmed:issn | 0020-7136 | lld:pubmed |
| pubmed-article:8567116 | pubmed:author | pubmed-author:PauliB UBU | lld:pubmed |
| pubmed-article:8567116 | pubmed:author | pubmed-author:HammerD ADA | lld:pubmed |
| pubmed-article:8567116 | pubmed:author | pubmed-author:el-SabbanM... | lld:pubmed |
| pubmed-article:8567116 | pubmed:author | pubmed-author:GoetzD JDJ | lld:pubmed |
| pubmed-article:8567116 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8567116 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:8567116 | pubmed:volume | 65 | lld:pubmed |
| pubmed-article:8567116 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8567116 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8567116 | pubmed:pagination | 192-9 | lld:pubmed |
| pubmed-article:8567116 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:meshHeading | pubmed-meshheading:8567116-... | lld:pubmed |
| pubmed-article:8567116 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8567116 | pubmed:articleTitle | Lu-ECAM-1-mediated adhesion of melanoma cells to endothelium under conditions of flow. | lld:pubmed |
| pubmed-article:8567116 | pubmed:affiliation | School of Chemical Engineering, Department of Pathology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. | lld:pubmed |
| pubmed-article:8567116 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8567116 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8567116 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8567116 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
