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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-3-7
|
| pubmed:databankReference | |
| pubmed:abstractText |
The recently cloned human breast and ovarian cancer susceptibility gene, BRCA1, is located on human chromosome 17q21. We have isolated murine genomic clones containing Brca1 as a first step in generating a mouse model for the loss of BRCA1 function. A mouse genomic library was screened using probes corresponding to exon 11 of the human BRCA1 gene. Two overlapping mouse clones were identified that hybridized to human BRCA1 exons 9-12. Sequence analysis of 1.4 kb of the region of these clones corresponding to part of human exon 11 revealed 72% nucleic acid identity but only 50% amino acid identity with the human gene. The longest of the mouse Brca1 genomic clones maps to chromosome 11D, as determined by two-color fluorescence in situ hybridization. The synteny to human chromosome 17 was confirmed by cohybridization with the mouse probe for the NF1-gene. This comparative study confirms that the relative location of the BRCA1 gene has been conserved between mice and humans.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0340-6717
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
97
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
256-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8566965-Amino Acid Sequence,
pubmed-meshheading:8566965-Animals,
pubmed-meshheading:8566965-BRCA1 Protein,
pubmed-meshheading:8566965-Breast Neoplasms,
pubmed-meshheading:8566965-Chromosome Mapping,
pubmed-meshheading:8566965-Cloning, Molecular,
pubmed-meshheading:8566965-Female,
pubmed-meshheading:8566965-Genetic Predisposition to Disease,
pubmed-meshheading:8566965-Humans,
pubmed-meshheading:8566965-In Situ Hybridization, Fluorescence,
pubmed-meshheading:8566965-Mice,
pubmed-meshheading:8566965-Molecular Sequence Data,
pubmed-meshheading:8566965-Neoplasm Proteins,
pubmed-meshheading:8566965-Ovarian Neoplasms,
pubmed-meshheading:8566965-Sequence Analysis, DNA,
pubmed-meshheading:8566965-Sequence Homology, Amino Acid,
pubmed-meshheading:8566965-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8566965-Transcription Factors
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
The murine homolog of the human breast and ovarian cancer susceptibility gene Brca1 maps to mouse chromosome 11D.
|
| pubmed:affiliation |
Diagnostic Development Branch, National Center for Human Genome Research/NIH, Bethesda, MD 20892-4470, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|