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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-3-6
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pubmed:abstractText |
A combination of signals transmitted through the antigen receptor, membrane-bound cell interaction molecules and cytokine receptors induces B cell proliferation and differentiation into immunoglobulin-secreting or memory cells. It has recently been suggested by Turner et al. (Cell 1994. 77: 297) that the complex changes in gene activities accompanying high levels of immunoglobulin secretion are under the common control of a master regulator, Blimp-1 (B lymphocyte-induced maturation protein). We show here that in naive mouse B cells stimulated with lipopolysaccharide (LPS) alone (which leads to high IgM production), Blimp-1 is highly expressed, while cells co-stimulated with LPS and anti-mu F(ab')2 show low levels of Blimp-1 mRNA and no longer secrete Ig. I gamma 1 sterile transcripts are, however, up-regulated after receptor co-ligation. Addition of interleukin (IL)-2 and IL-5 to LPS + anti-mu F(ab')2-treated primary B cells led to up-regulation of Blimp-1 and IgM secretion. Transfection of a Blimp-1 expression vector also induced IgM secretion. The data indicate that Blimp-1 is an important regulator of immunoglobulin secretion by primary B cells, and suggest that its level of expression may determine the differentiation to Ig-secreting plasma cells or entrance and maintenance in the memory pool.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Joining Region,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Prdm1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/anti-IgM
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
268-71
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8566078-Animals,
pubmed-meshheading:8566078-Antibodies, Anti-Idiotypic,
pubmed-meshheading:8566078-B-Lymphocytes,
pubmed-meshheading:8566078-Base Sequence,
pubmed-meshheading:8566078-Immunoglobulin Fab Fragments,
pubmed-meshheading:8566078-Immunoglobulin Joining Region,
pubmed-meshheading:8566078-Immunoglobulin M,
pubmed-meshheading:8566078-Immunosuppressive Agents,
pubmed-meshheading:8566078-Interleukin-2,
pubmed-meshheading:8566078-Interleukin-5,
pubmed-meshheading:8566078-Lipopolysaccharides,
pubmed-meshheading:8566078-Lymphocyte Activation,
pubmed-meshheading:8566078-Mice,
pubmed-meshheading:8566078-Mice, Inbred C57BL,
pubmed-meshheading:8566078-Mice, Inbred DBA,
pubmed-meshheading:8566078-Molecular Sequence Data,
pubmed-meshheading:8566078-RNA, Messenger,
pubmed-meshheading:8566078-Repressor Proteins,
pubmed-meshheading:8566078-Transcription, Genetic,
pubmed-meshheading:8566078-Transcription Factors,
pubmed-meshheading:8566078-Transfection,
pubmed-meshheading:8566078-Up-Regulation
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pubmed:year |
1996
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pubmed:articleTitle |
Blimp-1 overcomes the block in IgM secretion in lipopolysaccharide/anti-mu F(ab')2-co-stimulated B lymphocytes.
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pubmed:affiliation |
Institute for Virology and Immunobiology, University of Würzburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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