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rdf:type |
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lifeskim:mentions |
umls-concept:C0015301,
umls-concept:C0021920,
umls-concept:C0033684,
umls-concept:C0043393,
umls-concept:C0179400,
umls-concept:C0441843,
umls-concept:C0449432,
umls-concept:C0521451,
umls-concept:C0600688,
umls-concept:C1179435,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1705248
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pubmed:issue |
2
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pubmed:dateCreated |
1996-3-1
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pubmed:abstractText |
The yeast mitochondrial protein Suv3p is a putative NTP-dependent RNA helicase. Here we report that in cells lacking Suv3p, there is an approximately 50-fold increase in the excised form of the group I intron omega of the mitochondrial 31S rRNA gene. Surprisingly, little mature 21S rRNA accumulates in those cells; instead, unligated 21S rRNA exons appear. Intron overaccumulation could lead to spliced exon reopening via a reaction known to be catalyzed by group I introns in vitro. We also show that Suv3p is a functional component of a novel mitochondrial NTP-dependent 3'-to-5' exoribonuclease activity that can degrade group I intron RNAs. These findings account for group I intron overaccumulation in cells lacking Suv3p and define a novel function for putative RNA helicases in direct RNA degradation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DEAD-box RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Exoribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, mitochondrial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Nucleotidyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SUV3 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
199-209
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8565066-Base Sequence,
pubmed-meshheading:8565066-DEAD-box RNA Helicases,
pubmed-meshheading:8565066-DNA, Fungal,
pubmed-meshheading:8565066-Exons,
pubmed-meshheading:8565066-Exoribonucleases,
pubmed-meshheading:8565066-Fungal Proteins,
pubmed-meshheading:8565066-Introns,
pubmed-meshheading:8565066-Mitochondria,
pubmed-meshheading:8565066-Molecular Sequence Data,
pubmed-meshheading:8565066-RNA,
pubmed-meshheading:8565066-RNA, Fungal,
pubmed-meshheading:8565066-RNA, Ribosomal,
pubmed-meshheading:8565066-RNA Helicases,
pubmed-meshheading:8565066-RNA Nucleotidyltransferases,
pubmed-meshheading:8565066-RNA Splicing,
pubmed-meshheading:8565066-Recombinant Fusion Proteins,
pubmed-meshheading:8565066-Saccharomyces cerevisiae,
pubmed-meshheading:8565066-Saccharomyces cerevisiae Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
The DExH box protein Suv3p is a component of a yeast mitochondrial 3'-to-5' exoribonuclease that suppresses group I intron toxicity.
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pubmed:affiliation |
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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