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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1996-2-27
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pubmed:abstractText |
We describe here the generation and characterization of two human monoclonal IgM antibodies (UL-4F11 and UL-F6) reactive with HLA-B27. The monoclonal antibody (mAb) UL-4F11 is cytotoxic for peripheral mononuclear cells and, therefore, useful as typing reagent for HLA-B27 and HLA-B38. Protein chemistry showed that the mAb UL-4F11 precipitates HLA-B27 molecules. Epitope mapping analysis suggests that the amino acids 45, 67, 82 and 83 (alpha-1 domain) of the HLA-B27 sequence are necessary for mAb UL-4F11 reactivity. The mAb UL-F6 is suitable for complement dependent lysis of lymphoblastoid cell lines and stimulated peripheral blood mononuclear cells with HLA-B27 (B*2701, B*2702, B*2703, B*2705, B*2707), B13, B40 (60,61), B47 and B48 specificities. Its reactivity indicates that the amino acid valine in position 152 and glutamic acid in position 163 of the alpha-2 domain are crucial for the binding epitope.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0001-2815
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
305-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8560449-Amino Acid Sequence,
pubmed-meshheading:8560449-Animals,
pubmed-meshheading:8560449-Antibodies, Monoclonal,
pubmed-meshheading:8560449-Base Sequence,
pubmed-meshheading:8560449-Cell Line,
pubmed-meshheading:8560449-Cell Line, Transformed,
pubmed-meshheading:8560449-Epitope Mapping,
pubmed-meshheading:8560449-Female,
pubmed-meshheading:8560449-HLA-B27 Antigen,
pubmed-meshheading:8560449-Humans,
pubmed-meshheading:8560449-Immunoglobulin M,
pubmed-meshheading:8560449-Leukocytes, Mononuclear,
pubmed-meshheading:8560449-Mice,
pubmed-meshheading:8560449-Molecular Sequence Data,
pubmed-meshheading:8560449-Pregnancy,
pubmed-meshheading:8560449-Structure-Activity Relationship,
pubmed-meshheading:8560449-Tumor Cells, Cultured
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pubmed:year |
1995
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pubmed:articleTitle |
Characterization of two human IgM monoclonal antibodies reactive with HLA-B27.
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pubmed:affiliation |
Department of Transfusion Medicine, University of Ulm, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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