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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-2-26
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pubmed:abstractText |
Studies to determine if Toxoplasma gondii-specific human T cells lyse parasite-infected cells have yielded conflicting results. Furthermore, attempts to obtain human cytotoxic CD8+ T lymphocytes have been difficult because of the lack of a reproducible system for their generation. By using paraformaldehyde-fixed, T. gondii-infected peripheral blood mononuclear cells as antigen-presenting cells, we developed a method whereby T. gondii-specific T-cell lines can be reproducibly generated. Six T. gondii-specific T-cell lines were generated from an individual chronically infected with T. gondii. Cytofluorometric analysis of these lines revealed > 99% CD3+, 85 to 95% CD3+ alpha beta T-cell-receptor-positive (TCR+), 5 to 9% CD3+ gamma delta TCR+, 50 to 70% CD4+, and 20 to 40% CD8+ cells when cells were examined during the first 3 weeks of stimulation and >99% CD3+, >99% CD3+ alpha beta TCR+, < 1% CD3+ gamma delta TCR+, 20 to 40% CD4+, and 60 to 80% CD8+ cells when cells were examined between 5 and 11 weeks. Both CD4+ and CD8+ T cells had remarkable cytotoxic activity against T. gondii-infected target cells (30 to 50% specific Cr release at an effector-to-target ratio of 30:1) but not against uninfected target cells ( < 10% at an effector-to-target ratio of 30:1). Cytotoxic activity by the whole T-cell lines was not T. gondii strain specific. Whole T-cell lines were cytotoxic for target cells infected with the C56 and ME49 strains and the RH strain (which was used to infect peripheral blood mononuclear cells). T. gondii-specific T-cell lines displayed the predominant expression of V beta 7 TCR. The CDR3 regions of the V beta 7 TCRs of these T-cell lines showed a striking degree of sequence identity (oligoclonality). T-cell lines obtained by the method reporter here can be used to characterize functional activity of T-lymphocyte subsets in humans infected with T. gondii.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-1398756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-1460415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-1670604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-1679934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-17744024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-1908507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-1918963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-1940378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-1971829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-2112749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-2167101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-2479030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-2493090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-2786064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-2974063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-2997287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-3259601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-3511202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-3547029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-3818098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-4132992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-6238107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-7007520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-7615835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-7706828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-7995948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-8102155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-8274608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-8471767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8557337-8471768
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
176-81
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:8557337-Amino Acid Sequence,
pubmed-meshheading:8557337-Animals,
pubmed-meshheading:8557337-Base Sequence,
pubmed-meshheading:8557337-CD4-Positive T-Lymphocytes,
pubmed-meshheading:8557337-CD8-Positive T-Lymphocytes,
pubmed-meshheading:8557337-Cell Line,
pubmed-meshheading:8557337-Cloning, Molecular,
pubmed-meshheading:8557337-Cytotoxicity, Immunologic,
pubmed-meshheading:8557337-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:8557337-Flow Cytometry,
pubmed-meshheading:8557337-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:8557337-Humans,
pubmed-meshheading:8557337-Immunity, Innate,
pubmed-meshheading:8557337-Leukocytes, Mononuclear,
pubmed-meshheading:8557337-Molecular Sequence Data,
pubmed-meshheading:8557337-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8557337-Sequence Analysis, DNA,
pubmed-meshheading:8557337-T-Lymphocyte Subsets,
pubmed-meshheading:8557337-Toxoplasma
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pubmed:year |
1996
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pubmed:articleTitle |
Human CD4+ and CD8+ T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells.
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pubmed:affiliation |
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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