| pubmed-article:8555479 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8555479 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:8555479 | lifeskim:mentions | umls-concept:C0021760 | lld:lifeskim |
| pubmed-article:8555479 | lifeskim:mentions | umls-concept:C0079459 | lld:lifeskim |
| pubmed-article:8555479 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:8555479 | lifeskim:mentions | umls-concept:C0376152 | lld:lifeskim |
| pubmed-article:8555479 | lifeskim:mentions | umls-concept:C0927195 | lld:lifeskim |
| pubmed-article:8555479 | lifeskim:mentions | umls-concept:C2243049 | lld:lifeskim |
| pubmed-article:8555479 | lifeskim:mentions | umls-concept:C0947286 | lld:lifeskim |
| pubmed-article:8555479 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:8555479 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8555479 | pubmed:dateCreated | 1996-2-28 | lld:pubmed |
| pubmed-article:8555479 | pubmed:abstractText | The ability of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells (G-PBMCs) to induce secretion of cytokines in primary long-term marrow cultures (LTC) or in the human marrow stromal cell line HS23 was compared with that of marrow mononuclear cells. Equal numbers of G-PBMCs or marrow mononuclear cells were added to stromal cultures, supernatants were harvested at day 4 and levels of interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-2, IL-6, G-CSF, and tumor necrosis factor alpha (TNF alpha) were determined. G-PBMCs induced 21.4-fold higher levels of IL-6 and 12.5-fold higher levels of G-CSF in LTC cocultures compared with marrow mononuclear cells and induced 20.6-fold more IL-6 and 6.3-fold more G-CSF when added to HS23 cells. Experiments using sorted populations of CD20+, CD3+, and CD14+ cells showed that CD14+ cells within G-PBMCs were responsible for triggering the production of IL-6 and G-CSF. The effect did not require cell-cell contact and was inhibited when neutralizing antibodies to IL-1 alpha and IL-1 beta were used in combination. In these experiments, the greater stimulating ability of G-PBMCs is most likely attributable to the greater number of CD14+ cells in G-PBMCs (26.1+% +/- 2.3%) compared with marrow (2.5% +/- 0.8%), because equal numbers of CD14+ cells sorted from marrow and G-PBMCs showed comparable ability to induce IL-6 and G-CSF when placed directly on stromal cells. | lld:pubmed |
| pubmed-article:8555479 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8555479 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:8555479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8555479 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8555479 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8555479 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:8555479 | pubmed:author | pubmed-author:Torok-StorbBB | lld:pubmed |
| pubmed-article:8555479 | pubmed:author | pubmed-author:MielcarekMM | lld:pubmed |
| pubmed-article:8555479 | pubmed:author | pubmed-author:RoeckleinB... | lld:pubmed |
| pubmed-article:8555479 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8555479 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8555479 | pubmed:volume | 87 | lld:pubmed |
| pubmed-article:8555479 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8555479 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8555479 | pubmed:pagination | 574-80 | lld:pubmed |
| pubmed-article:8555479 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8555479 | pubmed:meshHeading | pubmed-meshheading:8555479-... | lld:pubmed |
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| pubmed-article:8555479 | pubmed:meshHeading | pubmed-meshheading:8555479-... | lld:pubmed |
| pubmed-article:8555479 | pubmed:meshHeading | pubmed-meshheading:8555479-... | lld:pubmed |
| pubmed-article:8555479 | pubmed:meshHeading | pubmed-meshheading:8555479-... | lld:pubmed |
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| pubmed-article:8555479 | pubmed:meshHeading | pubmed-meshheading:8555479-... | lld:pubmed |
| pubmed-article:8555479 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8555479 | pubmed:articleTitle | CD14+ cells in granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells induce secretion of interleukin-6 and G-CSF by marrow stroma. | lld:pubmed |
| pubmed-article:8555479 | pubmed:affiliation | Program in Transplantation Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA. | lld:pubmed |
| pubmed-article:8555479 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8555479 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8555479 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8555479 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8555479 | lld:pubmed |
