Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-2-20
pubmed:abstractText
Although many cells contain large amounts of InsP6, its metabolism and function is still largely unknown. In Dictyostelium lysates, the formation of InsP6 by sequential phosphorylation of inositol via Ins(3,4,6)P3 has been described [Stevens and Irvine (1990) Nature (London) 346, 580-583]; the second messenger Ins(1,4,5)P3 was excluded as a potential substrate or intermediate for InsP6 formation. However, we observed that mutant cells labelled in vivo with [3H]inositol showed altered labelling of both [3H]Ins(1,4,5)P3 and [3H]InsP6. In this report we demonstrate that Ins(1,4,5)P3 is converted into InsP6 in vitro by nucleus-associated enzymes, in addition to the previously described stepwise phosphorylation of inositol to InsP6 that occurs in the cytosol. HPLC analysis indicates that Ins(1,4,5)P3 is converted into InsP6 via sequential phosphorylation at the 3-, 6- and 2-positions. Ins[32P]P6, isolated from cells briefly labelled with [32P]Pi, was analysed using Paramecium phytase, which removes the phosphates of InsP6 in a specific sequence. The 6-position contained significantly more 32P radioactivity than the 4- or 5-positions, indicating that the 6-position is phosphorylated after the other two positions. The results from these in vivo and in vitro experiments demonstrate a metabolic route involving the phosphorylation of Ins(1,4,5)P3 via Ins(1,3,4,5)P4 and Ins(1,3,4,5,6)P5 to InsP6 in a nucleus-associated fraction of Dictyostelium cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-1310008, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-1312084, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-1316890, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-1325787, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-1325932, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-1628659, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-1646136, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-16666106, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-1958187, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2160808, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2171926, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2174555, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2174556, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2198472, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2375758, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2468517, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2539811, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2548474, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2550825, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2558121, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2843553, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-2910849, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-3277965, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-3683585, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-5530748, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-6249825, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-7737983, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-7778779, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-8001571, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-8156999, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-8343137, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-8382679, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-8394217, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-8440693, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554538-8554537
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
312 ( Pt 3)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
911-7
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Nucleus-associated phosphorylation of Ins(1,4,5)P3 to InsP6 in Dictyostelium.
pubmed:affiliation
Department of Biochemistry, University of Groningen, The Netherlands.
pubmed:publicationType
Journal Article