Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-2-20
pubmed:abstractText
Protein 4.1 is a major component of the erythrocyte membrane skeleton that promotes the interaction of spectrin with actin and links the resulting complex network to integral membrane proteins. Here we analyse the distribution of different 4.1 proteins within the nucleus of mammalian cells. Nuclear matrices have been prepared from Madin-Darby canine kidney (MDCK) and HeLa cells and protein fractions isolated at each step of the purifications have been analysed by immunoblotting using characterized polyclonal antibodies against protein 4.1. Two 4.1 polypeptides of M(r) approximately 135,000 and 175,000 are extracted after DNase I digestion and 0.25 M ammonium sulphate treatments, suggesting that they may be associated with chromatin. Interestingly, nuclear matrices isolated after DNase I digestion and sequential treatments with increasing ionic strength contain a third 4.1 polypeptide of M(r) approximately 75,000 (4.1p75), suggesting that it is a component of the nuclear matrix. Immunoblot analyses of nuclear matrices isolated from different cell types and species indicate that 4.1p75 is a common element of the nuclear matrix of mammalian cells. Moreover, 4.1p75 distributes to typical nuclear speckles which are enriched with the spliceosome assembly factor SC35, as revealed by double-label immunofluorescence analyses. Protein 4.1p75 might be an anchoring element of the nucleoskeleton, playing a role similar to that described for the erythroid protein 4.1 in red blood cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-1033834, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-1145202, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-14731494, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-1705562, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-1734026, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-1748285, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-1900457, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-1953652, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2004116, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2022644, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2137203, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2180926, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2211720, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2307700, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2307701, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2541917, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2668735, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2721591, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2958489, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2987966, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-2990913, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3031450, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3194408, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3196314, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3208744, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3277168, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3375238, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3410886, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3428276, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3462705, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3467321, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3474611, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3531202, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3700470, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-3949771, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-4214419, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-471052, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-563771, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6088992, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6091913, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6096007, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6177701, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6218995, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6230127, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6373017, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6589595, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6597817, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-6646237, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-7004645, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-7151171, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-7357619, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-7519621, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-7820866, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-7962048, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-8290569, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-8294510, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-8352961, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-8429050, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-8434260, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-8446901, http://linkedlifedata.com/resource/pubmed/commentcorrection/8554533-873992
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
312 ( Pt 3)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
871-7
pubmed:dateRevised
2011-6-20
pubmed:meshHeading
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