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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1996-2-22
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pubmed:databankReference | |
pubmed:abstractText |
A strain of Neurospora intermedia from China contains five prominent extragenomic mitochondrial plasmids: three linear elements called zhisi plasmids, and two circular plasmids, Harbin-1 and -2. In one subculture, levels of four plasmids (all three zhisis and Harbin-1) fell to undetectable values and two novel linear plasmids appeared, Harbin-L and -L2, as well as a new small circular plasmid, Harbin-0.9. Cross-hybridization of restriction fragments and DNA sequencing showed that the Harbin-L plasmid was composed of parts of the circular Harbin-1 plasmid and of one of the linear zhisi plasmids. A model is presented in which the Harbin-1 and zhisi plasmids are present within the same mitochondrion, and crossovers at two separate 7 bp sites of sequence identity effectively insert part of the circular Harbin-1 DNA into a zhisi linear plasmid, simultaneously deleting part of the zhisi element. The small plasmid Harbin-0.9 is a fragment of the Har-1 plasmid, and seems to be another product of the recombination process that created Har-L. Recombination of this type could have contributed to the wide array of mitochondrial plasmids found in natural populations of Neurospora.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0026-8925
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
|
pubmed:volume |
249
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
25-36
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8552030-Base Sequence,
pubmed-meshheading:8552030-DNA, Fungal,
pubmed-meshheading:8552030-DNA, Mitochondrial,
pubmed-meshheading:8552030-Molecular Sequence Data,
pubmed-meshheading:8552030-Neurospora,
pubmed-meshheading:8552030-Nucleic Acid Conformation,
pubmed-meshheading:8552030-Open Reading Frames,
pubmed-meshheading:8552030-Plasmids,
pubmed-meshheading:8552030-Recombination, Genetic,
pubmed-meshheading:8552030-Restriction Mapping,
pubmed-meshheading:8552030-Species Specificity
|
pubmed:year |
1995
|
pubmed:articleTitle |
Recombination between heterologous linear and circular mitochondrial plasmids in the fungus Neurospora.
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pubmed:affiliation |
Botany Department, University of British Columbia, Vancouver, Canada.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|