Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-2-20
|
| pubmed:abstractText |
The aim of this study was to examine the efficacy of semi-quantitative polymerase chain reaction (PCR) testing in cervical smears as an adjunct to cytological surveillance in a cohort of women with mild or moderate dyskaryosis. The study population comprised a group of 62 women who underwent twelve months of cytological and colposcopic surveillance as part of a larger randomised prospective study of women with mild and moderate dyskaryosis. Semi-quantitative PCR for HPV-16 DNA was carried out on the initial and twelve month study smears before a large loop excision of the transformation zone (LLETZ) was carried out. Smears from a control population which comprised 167 women without a history of abnormal cervical cytology who were attending family planning and general practitioner clinics for routine cervical smears were tested similarly. The presence of high or intermediate levels of HPV-16 DNA on both the initial and twelve month study smear was positively associated with the identification of cervical intraepithelial neoplasia (CIN) grades II or III in the LLETZ specimen (P = 0.01). While the combination of HPV-16 DNA testing with cytology on a repeat cervical smear improved the selection of women with underlying CIN II/III, there was still a false negative rate of 53%. Twenty-nine women had 'low risk' levels of HPV-16 DNA and mild dyskaryosis or less on both repeat smears indicating suitability for surveillance, but in fact 34% of them had CIN II/III. This study supports the finding reported previously of an association between high and intermediate levels of HPV-16 DNA and CIN II/III.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0146-6615
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
47
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8551261-Adult,
pubmed-meshheading:8551261-Cervical Intraepithelial Neoplasia,
pubmed-meshheading:8551261-Cervix Uteri,
pubmed-meshheading:8551261-Cohort Studies,
pubmed-meshheading:8551261-DNA, Viral,
pubmed-meshheading:8551261-Female,
pubmed-meshheading:8551261-Humans,
pubmed-meshheading:8551261-Papillomaviridae,
pubmed-meshheading:8551261-Pilot Projects,
pubmed-meshheading:8551261-Polymerase Chain Reaction,
pubmed-meshheading:8551261-Prospective Studies,
pubmed-meshheading:8551261-Sensitivity and Specificity,
pubmed-meshheading:8551261-Uterine Cervical Neoplasms,
pubmed-meshheading:8551261-Vaginal Smears
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Serial quantitation of HPV-16 in the smears of women with mild and moderate dyskaryosis.
|
| pubmed:affiliation |
Wellbeing Centre for the Prevention of Cervical Cancer, Aberdeen Royal Infirmary, University of Aberdeen, Scotland.
|
| pubmed:publicationType |
Journal Article
|