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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-2-20
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pubmed:databankReference |
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pubmed:abstractText |
Human involucrin whose gene transcription is directed by a 2456-nucleotide (nt) 5'-noncoding region is a structural component of the epithelial cornified layer. Transient transfection assays demonstrated that this region is transcriptionally active in multiplying keratinocytes and is enhanced by 2 mM CaCl2 treatment. Calcium-independent transcriptional activity and the interaction with the AP-1 transcriptional factor was located on the proximal part (nt -159 to -1) of the 5'-noncoding region. However, CaCl2 responsiveness was mapped to a distal 1185-nt fragment (nt -2456 to -1272). Moreover, this fragment potentiated the Herpes simplex thymidine kinase promoter in normal keratinocytes and is responsive to calcium treatment in a cell type-specific manner. Interestingly, the absence of a 491-nt fragment located between the two enhancer domains (nt -651 to -160) resulted in transcriptional activation in multiplying keratinocytes. This fragment interacts with AP-1 and the YY1 transcriptional silencer. It is concluded that human involucrin 5'-noncoding region contains at least three regulatory domains, a distal CaCl2-responsive enhancer, a putative transcriptional silencer (that interacts with AP-1 and YY1), and a proximal enhancer/promoter (that interacts with AP-1). Thus, this study demonstrates the presence of particular transcriptional factors can potentially regulate the human involucrin expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
512-20
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8550612-Animals,
pubmed-meshheading:8550612-Base Sequence,
pubmed-meshheading:8550612-Cattle,
pubmed-meshheading:8550612-Cells, Cultured,
pubmed-meshheading:8550612-DNA,
pubmed-meshheading:8550612-DNA Footprinting,
pubmed-meshheading:8550612-DNA-Binding Proteins,
pubmed-meshheading:8550612-Enhancer Elements, Genetic,
pubmed-meshheading:8550612-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:8550612-HeLa Cells,
pubmed-meshheading:8550612-Humans,
pubmed-meshheading:8550612-Keratinocytes,
pubmed-meshheading:8550612-Molecular Sequence Data,
pubmed-meshheading:8550612-Promoter Regions, Genetic,
pubmed-meshheading:8550612-Protein Binding,
pubmed-meshheading:8550612-Protein Precursors,
pubmed-meshheading:8550612-Transcription, Genetic,
pubmed-meshheading:8550612-Transcription Factor AP-1,
pubmed-meshheading:8550612-Transcription Factors,
pubmed-meshheading:8550612-Transfection,
pubmed-meshheading:8550612-YY1 Transcription Factor
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pubmed:year |
1996
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pubmed:articleTitle |
Transcriptional analysis of the 5'-noncoding region of the human involucrin gene.
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pubmed:affiliation |
Departamento de Genetica y Biologia Molecular, Centro de Investigacion y Estudios Avanzados del Instituto Politecnico Nacional, Mexico, Distrito Federal, Mexico.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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