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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-2-20
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pubmed:abstractText |
sigma E and sigma K are sporulation-specific sigma factors of Bacillus subtilis that are synthesized as inactive proproteins. Pro-sigma E and pro-sigma K are activated by the removal of 27 and 20 amino acids, respectively, from their amino termini. To explore the properties of the precursor-specific sequences, we exchanged the coding elements for these domains in the sigma E and sigma K structural genes and determined the properties of the resulting chimeric proteins in B. subtilis. The pro-sigma E-sigma K chimera accumulated and was cleaved into active sigma K, while the pro-sigma K-sigma E fusion protein failed to accumulate and is likely unstable in B. subtilis. A fusion of the sigE "pro" sequence to an unrelated protein (bovine rhodanese) also formed a protein that was cleaved by the pro-sigma E processing apparatus. The data suggest that the sigma E pro sequence contains sufficient information for pro-sigma E processing as well as a unique quality needed for sigma E accumulation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-1538747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-1624450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-1691789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-1900494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-1948031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-2002017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-2124700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-2163341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-2439490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-2461938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-2536191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-2841290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-3104904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-3125985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-3918005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-4956288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-6438529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-7883171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-7892217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-8226681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-8320224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550479-8464402
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
546-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8550479-Amino Acid Sequence,
pubmed-meshheading:8550479-Animals,
pubmed-meshheading:8550479-Bacillus subtilis,
pubmed-meshheading:8550479-Base Sequence,
pubmed-meshheading:8550479-Cattle,
pubmed-meshheading:8550479-DNA, Bacterial,
pubmed-meshheading:8550479-Genes, Bacterial,
pubmed-meshheading:8550479-Molecular Sequence Data,
pubmed-meshheading:8550479-Protein Precursors,
pubmed-meshheading:8550479-Protein Processing, Post-Translational,
pubmed-meshheading:8550479-Recombinant Fusion Proteins,
pubmed-meshheading:8550479-Sigma Factor,
pubmed-meshheading:8550479-Spores, Bacterial,
pubmed-meshheading:8550479-Thiosulfate Sulfurtransferase,
pubmed-meshheading:8550479-Transcription Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Exchange of precursor-specific elements between Pro-sigma E and Pro-sigma K of Bacillus subtilis.
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pubmed:affiliation |
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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