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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-2-22
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated a sorbitol-negative mutant of Streptococcus mutans GS-5 following random mutagenesis with plasmid pVA891 clone banks. This mutant did not metabolize sorbitol anaerobically but did so aerobically. A 10-kb chromosomal DNA fragment flanking the pVA891 insertion was deleted in this mutant. The corresponding region from the parental strain GS-5 was then recovered by a marker rescue method with Escherichia coli. The pyruvate formate-lyase gene, pfl, was identified within a 3-kb PstI-XbaI fragment located in the middle of the deleted region of the chromosome, and its inactivation in S. mutans produced the same sorbitol-negative phenotype. Nucleotide sequence analysis of the pfl gene revealed a 2.3-kb open reading frame (ORF) preceded by potential ribosome-binding and promoter-like sequences. The ORF specified a putative protein of 775 amino acid residues with a calculated molecular weight of 87,533. The amino acid sequence deduced from the ORF exhibited significant similarity to that of the E. coli pfl gene.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-1176435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-1310545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-1325457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-1624103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-2015910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-2553398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-2651404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-3053170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-3323803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-3540569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-3818089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-3965391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-4513107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-4531871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-6223884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-6235151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-6319229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-6369325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-6811549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-7689539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-7873518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-8254304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8550181-8336109
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
385-91
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8550181-Acetyltransferases,
pubmed-meshheading:8550181-Amino Acid Sequence,
pubmed-meshheading:8550181-Base Sequence,
pubmed-meshheading:8550181-Cloning, Molecular,
pubmed-meshheading:8550181-Genes, Bacterial,
pubmed-meshheading:8550181-Molecular Sequence Data,
pubmed-meshheading:8550181-Mutation,
pubmed-meshheading:8550181-Open Reading Frames,
pubmed-meshheading:8550181-Streptococcus mutans
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pubmed:year |
1996
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pubmed:articleTitle |
Cloning and sequence analysis of the pfl gene encoding pyruvate formate-lyase from Streptococcus mutans.
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pubmed:affiliation |
Department of Biochemistry, Tokyo Dental College, Chiba City, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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