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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1996-2-21
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pubmed:abstractText |
Defined segments of the gag polyprotein and transmembrane envelope glycoprotein from Maedi-visna virus were expressed as glutathione S-transferase fusion proteins in Escherichia coli and evaluated singly and in combination for use in an enzyme-linked immunosorbent assay (ELISA). Two hundred sixty field serum specimens from 15 sheep flocks were tested in parallel with recombinant and whole-virus antigens, and the relative sensitivities and specificities of the recombinant antigens were calculated. When the recombinant gag and transmembrane proteins were used in combination, a sensitivity of 97.4% and a specificity of 99.4% relative to whole-virus antigen were observed, indicating the utility of these proteins in diagnostic testing.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-1314572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-1653261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-1847257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-2159018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-2174295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-2174296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-2325206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-2410140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-2539693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-2824836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-2988088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-3010411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-3324469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-6287705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-6307878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-7929780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-8067051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-8091643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-8382414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548546-8507695
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1071-412X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
631-3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1995
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pubmed:articleTitle |
Evaluation of two recombinant Maedi-visna virus proteins for use in an enzyme-linked immunosorbent assay for the detection of serum antibodies to ovine lentiviruses.
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pubmed:affiliation |
Health of Animals Laboratory, Agriculture Canada, Sackville, New Brunswick, Canada.
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pubmed:publicationType |
Journal Article
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