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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1996-2-21
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pubmed:abstractText |
Antibodies against reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) have been detected in seropositive subjects by immunoprecipitation, Western immunoblotting, and neutralization assay. Recently, we noticed that the antibodies against RT stabilized RT upon heat inactivation, and we have developed a stabilization assay of RT antibody. Briefly, the RT of HIV-1 is completely inactivated by incubation at 56 degrees C for 20 min, but this inactivation is inhibited in the presence of a specific antibody directed against this molecule. We examined the specificity and clinical significance of this stabilization assay. HIV-1 antibody-positive sera stabilized HIV-1 RT but not HIV-2 RT, whereas half of these sera cross-neutralized HIV-2 RT. Antibody titers against RT determined by the neutralization assay and the stabilization assay were compared with clinical characteristics. Antibodies against HIV-1 RT were much more frequently detected by the stabilization assay than by the neutralization assay. Statistically significant associations were found between stabilizing antibody titer and CD4+ cell number in peripheral blood of patients and also between antibody titer and CD4+/CD8+ ratios. These results indicate that our new stabilization assay to detect specific antibodies against RT of HIV-1 is useful as a clinical marker of infection and progress of the disease.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-1379577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2418504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2419583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2435004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2448336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2582414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2984774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2986290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2986291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2989707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-2994223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-3007413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-3024760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-3146268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-3258005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-6095094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-6189183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-6200935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-6200936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-6205277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-6206563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8548538-6261256
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1071-412X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
583-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8548538-AIDS Serodiagnosis,
pubmed-meshheading:8548538-Adult,
pubmed-meshheading:8548538-Antibody Specificity,
pubmed-meshheading:8548538-Blotting, Western,
pubmed-meshheading:8548538-Enzyme Activation,
pubmed-meshheading:8548538-Enzyme Stability,
pubmed-meshheading:8548538-Female,
pubmed-meshheading:8548538-HIV Antibodies,
pubmed-meshheading:8548538-HIV-1,
pubmed-meshheading:8548538-Hot Temperature,
pubmed-meshheading:8548538-Humans,
pubmed-meshheading:8548538-Male,
pubmed-meshheading:8548538-Neutralization Tests,
pubmed-meshheading:8548538-RNA-Directed DNA Polymerase
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pubmed:year |
1995
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pubmed:articleTitle |
Characteristics and clinical significance of a stabilization assay to detect specific antibodies to reverse transcriptase of human immunodeficiency virus.
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pubmed:affiliation |
Division of Biomedical Research, Kitasato Institute Hospital, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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