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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1996-2-14
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pubmed:abstractText |
Although several alpha-adrenergic receptor genes are expressed in the rat kidney, their expression in the renal vasculature has not been studied. Since pharmacological studies have suggested that an alpha 1B-adrenergic receptor may mediate renal vasoconstriction, we studied the expression of alpha 1B-adrenergic receptors in renal microvessels, from 10- to 14-week-old male spontaneously hypertensive rats (SHR) and their normotensive control, the Wistar-Kyoto rat (WKY). In these microvessels, isolated by perfusion with iron, alpha 1B-adrenergic receptor mRNA levels (by ribonuclease protection assay) were similar in SHR and WKY rats. Photo-affinity labeling with [125I]-arylazidoprazosin demonstrated the presence of alpha 1B-adrenergic receptor protein. Maximum receptor density (determined by 3H-prazosin binding: Bmax 59.8 +/- 4.1 and 58.7 +/- 4.3; Kd 0.48 +/- 0.05 nM and 0.31 +/- 0.06 nM in SHR and WKY, respectively) and chloroethylclonidine (CEC)-sensitive binding sites (determined by [125I]-(2-beta(4-hydroxyphenyl)-ethylaminomethyl)-tetralone binding) (125I-HEAT) were similar in SHR and WKY rats. There are two novel findings in these studies: (1) the alpha 1B-adrenergic receptor gene is expressed in renal microvessels of WKY and SHR; (2) alpha 1B-adrenergic receptor gene expression in renal microvessels is not altered in adult SHR. The failure to down-regulate expression of the alpha 1B-adrenergic receptor at the mRNA and protein level in the SHR could result in persistence of alpha 1B-adrenergic receptor effects and contribute to the increased vascular resistance in hypertension.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Affinity Labels,
http://linkedlifedata.com/resource/pubmed/chemical/Alkylating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Azides,
http://linkedlifedata.com/resource/pubmed/chemical/Clonidine,
http://linkedlifedata.com/resource/pubmed/chemical/Prazosin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, alpha,
http://linkedlifedata.com/resource/pubmed/chemical/azidoprazosin,
http://linkedlifedata.com/resource/pubmed/chemical/chlorethylclonidine
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0085-2538
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1412-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8544397-Affinity Labels,
pubmed-meshheading:8544397-Alkylating Agents,
pubmed-meshheading:8544397-Animals,
pubmed-meshheading:8544397-Azides,
pubmed-meshheading:8544397-Binding Sites,
pubmed-meshheading:8544397-Blood Vessels,
pubmed-meshheading:8544397-Clonidine,
pubmed-meshheading:8544397-Male,
pubmed-meshheading:8544397-Microcirculation,
pubmed-meshheading:8544397-Prazosin,
pubmed-meshheading:8544397-RNA, Messenger,
pubmed-meshheading:8544397-Radioligand Assay,
pubmed-meshheading:8544397-Rats,
pubmed-meshheading:8544397-Rats, Inbred SHR,
pubmed-meshheading:8544397-Rats, Inbred WKY,
pubmed-meshheading:8544397-Receptors, Adrenergic, alpha,
pubmed-meshheading:8544397-Renal Circulation
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pubmed:year |
1995
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pubmed:articleTitle |
Alpha 1B-adrenergic receptors in rat renal microvessels.
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pubmed:affiliation |
Department of Pediatrics, Georgetown University Children's Medical Center, Washington, D.C., USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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