Linked Life Data

8541298

Source:http://linkedlifedata.com/resource/pubmed/id/8541298

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-2-12
pubmed:abstractText
Internal volume is a very sensitive parameter of vesicle morphology. Measurement of captured volumes by solute entrapment is legitimate for most types of vesicles (Perkin, W.R. et al. (1993) Chem. Phys. Lipids 64, 197-217). In this study chloride was selected as the most convenient marker ion because the ubiquity of Cl- in physiological buffers eliminates prelabeling with exogenous markers and because minute concentrations of trapped chloride are well detectable in the presence of large extravesicular nitrate concentrations. Perfect exchange of external chloride for nitrate was shown to be accomplished by gel filtration, dialysis, or sucrose gradient flotation-but only after significant technical improvements and/or elimination of experimental pitfalls. Reliability was cross-checked by simultaneous entrapment of Cl- and K+. Diafiltration and ion exchange chromatography appeared inapplicable for exchange of extravesicular salt. When a representative variety of vesicle preparations was analyzed for internal volume (as well as for external surface and size) unexpected features of vesicle morphology were discovered. This emphasizes the genuine role of macroscopic vesicle characterization in complementing information from electron microscopy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
1240
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
266-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Measurement of intravesicular volumes by salt entrapment.
pubmed:affiliation
Institute of Biophysics, J. Kepler University, Linz, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't