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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1996-2-6
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pubmed:abstractText |
Pfs16 is a sexual stage/sporozoite-specific antigen of Plasmodium falciparum and is a potential candidate for a sporozoite-neutralizing vaccine. To obtain more information on the function of Pfs16 and to investigate its role during transmission and hepatocyte invasion, immunization experiments were performed with both a Pfs16-specific recombinant vaccinia virus and virus-like particles produced in yeast composed of the hepatitis B surface antigen (HBsAg) and antigen Pfs16 fused to HBsAg. Upon transformation of yeast cells, harbouring a genomic copy of the HBsAg gene, with a plasmid carrying the fusion gene Pfs16-HBsAg (Pfs16-S) virus-like hybrid particles composed of HBsAg and Pfs16-S were formed of a size similar to those present in human sera after infection with the hepatitis B virus. Cells infected with recombinant Pfs16 vaccinia virus synthesized a polypeptide of approx. 16 kDa that reacted with a Pfs16-specific polyclonal antibody. Animals vaccinated with the yeast hybrid particles and/or recombinant vaccinia virus both produced Pfs16-specific antibodies. These antibodies showed no transmission-blocking activity, but they efficiently diminished or abolished in vitro invasion of sporozoites into human hepatoma cells (HepG2-A16) and primary human hepatocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Surface Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Malaria Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pfs16 protein, Plasmodium falciparum,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0166-6851
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
179-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8538688-Amino Acid Sequence,
pubmed-meshheading:8538688-Animals,
pubmed-meshheading:8538688-Anopheles,
pubmed-meshheading:8538688-Antibodies, Protozoan,
pubmed-meshheading:8538688-Antibody Specificity,
pubmed-meshheading:8538688-Antigens, Protozoan,
pubmed-meshheading:8538688-Carcinoma, Hepatocellular,
pubmed-meshheading:8538688-Cells, Cultured,
pubmed-meshheading:8538688-Female,
pubmed-meshheading:8538688-Hepatitis B Surface Antigens,
pubmed-meshheading:8538688-Humans,
pubmed-meshheading:8538688-Insect Vectors,
pubmed-meshheading:8538688-Liver,
pubmed-meshheading:8538688-Liver Neoplasms,
pubmed-meshheading:8538688-Malaria Vaccines,
pubmed-meshheading:8538688-Membrane Proteins,
pubmed-meshheading:8538688-Mice,
pubmed-meshheading:8538688-Molecular Sequence Data,
pubmed-meshheading:8538688-Plasmodium falciparum,
pubmed-meshheading:8538688-Rabbits,
pubmed-meshheading:8538688-Recombinant Fusion Proteins,
pubmed-meshheading:8538688-Saccharomyces cerevisiae,
pubmed-meshheading:8538688-Tumor Cells, Cultured,
pubmed-meshheading:8538688-Vaccines, Synthetic,
pubmed-meshheading:8538688-Vaccinia virus
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pubmed:year |
1995
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pubmed:articleTitle |
Induction of Plasmodium falciparum sporozoite-neutralizing antibodies upon vaccination with recombinant Pfs16 vaccinia virus and/or recombinant Pfs16 protein produced in yeast.
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pubmed:affiliation |
Department of Molecular Biology, Faculty of Science, University of Nijmegen, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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