Linked Life Data

8538672

Source:http://linkedlifedata.com/resource/pubmed/id/8538672

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-2-7
pubmed:abstractText
We investigated whether malignant hyperthermia (MH)-related contractile abnormalities, such as lowered contractile threshold, were expressed in MH-susceptible (MHS) immature muscles and myotubes. Muscles from neonatal piglets homozygous for Arg615 (normal) or for Cys615 (MHS) ryanodine receptor alleles, and heterozygotes were used. Intact cell bundles from piglet muscles generally were similar in contractile properties to adult muscles of the same genotype. Thresholds for K contractures in normal, heterozygous, and MHS piglet muscles (40 mmol/L, 25 mmol/L and 15 mmol/L K+, respectively) differed significantly. Cultured myotubes were subjected to a series of square pulses of varying strengths (-50 to +50 mV) and durations (25-300 ms) using whole cell patch-clamp techniques. Threshold for contraction differed significantly among the three genotypes, for example, with 300 msec pulses thresholds were -6.9 +/- 0.9, -12.4 +/- 1.6, and -22.6 +/- 2.6 mV for normal, heterozygous and MHS myotubes, respectively. Thus a significantly lower than normal threshold for contraction was expressed in MHS and heterozygous piglet muscles and myotubes. Further, these developmentally immature preparations are likely to express other differences characteristic of adult MHS muscles, and thus provide suitable preparations for clinically relevant studies of MH-related cellular abnormalities.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0148-639X
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
68-73
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Porcine malignant hyperthermia: genotype and contractile threshold of immature muscles.
pubmed:affiliation
Department of Veterinary PathoBiology, University of Minnesota, St. Paul, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't