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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-2-7
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D16541,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D16844,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L19140,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M95596,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M97767,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M98338,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U28657,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X17329,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X17330,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59596,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X64696,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X64697,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X64698,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X64699,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X64700,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X69490,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z17410,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z24824,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z28527
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pubmed:abstractText |
Titin is an approximately 3 MDa protein that spans from the M- to the Z-line in the sarcomeres of vertebrate striated muscle. The protein is presumably encoded by unusually large mRNAs of 70-80 kb. Although titin has been studied by several laboratories, barely more than half of the cDNA sequence (approximately 45 kb) has been published, most of it obtained from the A-band and M-line region (corresponding to the C-terminal half of the molecule). A special cDNA library was constructed using size selected total RNA from adult rabbit cardiac muscle in order to obtain sequence data from titin's unknown N-terminal region. A monoclonal antibody (T12), which binds to an epitope close to the Z-line, was used to identify initial cDNA clones. Additional overlapping clones were isolated and sequenced yielding a 5.4 kb contig. The encoded polypeptide contains 16 Type-II domains and four unique intervening segments. Polyclonal sera, raised against an expressed protein fragment encoded by the 5' end of the contig, strongly stained the Z-line of myofibrils of different species. However, the sequence of this fragment is 83% identical at the amino acid level with the previously reported C-terminal (i.e. M-line) end of chicken embryonic skeletal muscle titin. The expressed protein fragment could be phosphorylated in vitro by embryonic skeletal muscle extract and by the purified proline-directed kinase ERK1, presumably at the xSPxR recognition sites located in the first interdomain segment.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9533
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
108 ( Pt 9)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3029-37
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8537442-Amino Acid Sequence,
pubmed-meshheading:8537442-Animals,
pubmed-meshheading:8537442-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:8537442-DNA, Complementary,
pubmed-meshheading:8537442-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:8537442-Mitogen-Activated Protein Kinases,
pubmed-meshheading:8537442-Molecular Sequence Data,
pubmed-meshheading:8537442-Muscle Proteins,
pubmed-meshheading:8537442-Myocardium,
pubmed-meshheading:8537442-Peptide Fragments,
pubmed-meshheading:8537442-Phosphorylation,
pubmed-meshheading:8537442-Protein Kinases,
pubmed-meshheading:8537442-Rabbits,
pubmed-meshheading:8537442-Sequence Homology, Amino Acid
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pubmed:year |
1995
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pubmed:articleTitle |
Characterization of a 5.4 kb cDNA fragment from the Z-line region of rabbit cardiac titin reveals phosphorylation sites for proline-directed kinases.
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pubmed:affiliation |
Department of Pediatrics, Waisman Center, University of Wisconsin-Madison 53705, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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