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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
52
|
| pubmed:dateCreated |
1996-2-8
|
| pubmed:databankReference | |
| pubmed:abstractText |
The immediate early gene, junB, is induced by interleukin-6 (IL-6) in plasmacytomas. In order to identify enhancers that mediate this effect, we cloned upstream and downstream sequences flanking the gene into a luciferase reporter gene vector containing the junB promoter and evaluated the IL-6 inducibility of these sequences by transient expression in an IL-6-dependent plasmacytoma cell line. Although a 6.5-kilobase fragment of upstream flanking sequence did not increase the IL-6 inducibility of the junB promoter, a 222-base pair fragment was identified in 2.1 kilobases of down-stream flanking sequence that both up-regulates the promoter and confers inducibility by IL-6. Point mutation of an acute phase response factor (APRF) site within this region significantly reduced up-regulation of the promoter in cells grown continuously in IL-6, as well as inducibility upon restimulation of cells with IL-6 after withdrawal from the growth factor. Point mutation of an NF-kappa B site sharing five nucleotides with the APRF site reduced up-regulation of the promoter but not inducibility by IL-6, whereas mutation of two other NF-kappa B sites in the 222-base pair fragment had no effect on expression. Western blotting of nuclear proteins purified by DNA affinity chromatography revealed inducible binding of Stat3 and constitutive binding of NF-kappa B p65 to the APRF/NF-kappa B site.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
29
|
| pubmed:volume |
270
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
31129-35
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8537375-Animals,
pubmed-meshheading:8537375-Base Sequence,
pubmed-meshheading:8537375-Binding Sites,
pubmed-meshheading:8537375-DNA-Binding Proteins,
pubmed-meshheading:8537375-Enhancer Elements, Genetic,
pubmed-meshheading:8537375-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8537375-Genes, jun,
pubmed-meshheading:8537375-Interleukin-6,
pubmed-meshheading:8537375-Mice,
pubmed-meshheading:8537375-Mice, Inbred BALB C,
pubmed-meshheading:8537375-Molecular Sequence Data,
pubmed-meshheading:8537375-NF-kappa B,
pubmed-meshheading:8537375-Nuclear Proteins,
pubmed-meshheading:8537375-Oligodeoxyribonucleotides,
pubmed-meshheading:8537375-Plasmacytoma,
pubmed-meshheading:8537375-Promoter Regions, Genetic,
pubmed-meshheading:8537375-STAT3 Transcription Factor,
pubmed-meshheading:8537375-Trans-Activators
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
An acute phase response factor/NF-kappa B site downstream of the junB gene that mediates responsiveness to interleukin-6 in a murine plasmacytoma.
|
| pubmed:affiliation |
Division of Monoclonal Antibodies, Center for Biologics Evaluation and Research, Bethesda, Maryland 20892, USA.
|
| pubmed:publicationType |
Journal Article
|