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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1996-2-7
|
| pubmed:abstractText |
Monoclonal antibody (HS-63) raised in mice against human ejaculated sperm, polyclonal antibodies raised in rabbits against the cognate mouse testicular antigen (MSA-63; or Fab) and polyclonal antibodies raised in the rabbit against recombinant fusion proteins (GST-63) showed acrosomal localization in permeabilized rhesus monkey and human ejaculated sperm. Tail localization of the cognate primate sperm antigen (PSA-63) was also seen with intact MSA-63 antibodies and Fab fragments. The ability of these antibodies to inhibit sperm binding to the zona pellucida was measured with hemizona binding assays (HZAs). HS-63 (1.2 mg/ml) inhibited rhesus monkey sperm binding (mean +/- SEM) to homologous hemizonae (treatment, 15.5 +/- 3.3; control, 58.9 +/- 9.4; P < 0.025), whereas comparable concentrations of protein from nonimmunized mouse preparations were inactive (ascites fluid, 67.6 +/- 43.5; no ascites fluid, 72.0 +/- 44.6). Intact MSA-63 antibodies inhibited (up to 99%) monkey sperm-zona binding in a concentration-dependent manner. Moreover, inhibition in this case by intact MSA-63 antibody was limited to capacitated sperm. Similarly, intact MSA-63 antibodies inhibited (up to 85%) human sperm binding to homologous zonae in an antibody concentration-dependent manner. Fab fragments derived from MSA-63, when present in insemination mixtures (0.5 mg/ml), inhibited (P < 0.01) primate sperm binding to homologous hemizonae (monkey, 9.6 +/- 3; human sperm 9.4 +/- 2) compared with matched hemizona controls (monkey, 117 +/- 29; human, 20.4 +/- 3). Furthermore, rhesus monkey sperm-zona binding was reduced by 84% in the presence of rabbit anti-GST-63 antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ACRV1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Acrv1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0196-3635
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
318-26
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8537249-Acrosome,
pubmed-meshheading:8537249-Animals,
pubmed-meshheading:8537249-Antibody Specificity,
pubmed-meshheading:8537249-Antigens,
pubmed-meshheading:8537249-Contraception, Immunologic,
pubmed-meshheading:8537249-Female,
pubmed-meshheading:8537249-Humans,
pubmed-meshheading:8537249-Macaca mulatta,
pubmed-meshheading:8537249-Male,
pubmed-meshheading:8537249-Membrane Proteins,
pubmed-meshheading:8537249-Mice,
pubmed-meshheading:8537249-Proteins,
pubmed-meshheading:8537249-Rabbits,
pubmed-meshheading:8537249-Species Specificity,
pubmed-meshheading:8537249-Sperm Agglutination,
pubmed-meshheading:8537249-Sperm Capacitation,
pubmed-meshheading:8537249-Sperm-Ovum Interactions,
pubmed-meshheading:8537249-Zona Pellucida
|
| pubmed:articleTitle |
Functional characterization of the primate sperm acrosomal antigen (PSA-63).
|
| pubmed:affiliation |
Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton 97006, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|