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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-2-5
|
| pubmed:abstractText |
To test the hypothesis that insulin-like growth factor I (IGF-I) regulates mammary gland development and lactation, the expression of both human (h) IGF-I and des(1-3)hIGF-I was targeted to the mammary gland in transgenic mice using a novel exon replacement strategy and the rat whey acidic protein (rWAP) gene regulatory sequences. Both transgenes expressed a 0.7-kilobase messenger RNA (mRNA). The abundance of WAP-IGE-I and WAP-DES mRNA on day 10 of lactation ranged from 0.2-1.0% and 0.2-13% of the endogenous mouse WAP mRNA, respectively. For WAP-DES mice, transgene expression was greatest from midpregnancy throughout lactation. Western blot analysis showed the presence of correctly processed hIGF-I in milk from these transgenic mice. This hIGF-I was capable of stimulating protein synthesis in cultured rat L6 myoblasts. Ligand blotting indicated changes in mammary gland secretion of IGFBP in response to WAP-DES expression. Histological analysis of mammary tissue from mice overexpressing des(1-3)hIGF-I showed incomplete mammary involution, ductile hypertrophy, and loss of secretory lobules associated with increased deposition of collagen. These changes are believed to occur through autocrine and paracrine effects of des(1-3)-hIGF-I on both epithelial and stromal cells.
|
| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Milk Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/insulin-like growth factor 1...,
http://linkedlifedata.com/resource/pubmed/chemical/whey acidic proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0013-7227
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
137
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
321-30
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8536631-Animals,
pubmed-meshheading:8536631-Base Sequence,
pubmed-meshheading:8536631-Female,
pubmed-meshheading:8536631-Gene Expression Regulation,
pubmed-meshheading:8536631-Humans,
pubmed-meshheading:8536631-Insulin-Like Growth Factor Binding Proteins,
pubmed-meshheading:8536631-Insulin-Like Growth Factor I,
pubmed-meshheading:8536631-Lactation,
pubmed-meshheading:8536631-Mammary Glands, Animal,
pubmed-meshheading:8536631-Mice,
pubmed-meshheading:8536631-Mice, Transgenic,
pubmed-meshheading:8536631-Milk Proteins,
pubmed-meshheading:8536631-Molecular Sequence Data,
pubmed-meshheading:8536631-Oligonucleotide Probes,
pubmed-meshheading:8536631-Peptide Fragments,
pubmed-meshheading:8536631-RNA, Messenger,
pubmed-meshheading:8536631-Rats,
pubmed-meshheading:8536631-Tissue Distribution
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Targeted expression of des(1-3) human insulin-like growth factor I in transgenic mice influences mammary gland development and IGF-binding protein expression.
|
| pubmed:affiliation |
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|