Linked Life Data

8534853

Source:http://linkedlifedata.com/resource/pubmed/id/8534853

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-2-6
pubmed:databankReference
pubmed:abstractText
ADP-ribosylation factor (ARF) is a highly conserved, low molecular mass (ca. 21 kDa) GTP-binding protein that has been implicated in vesicle trafficking and signal transduction in yeast and mammalian cells. However, little is known of ARF in plant systems. A putative ARF polypeptide was identified in subcellular fractions of the green alga Chlamydomonas reinhardtii, based on [32P]GTP binding and immunoblot assays. A cDNA clone was isolated from Chlamydomonas (Arf1), which encodes a 20.7 kDa protein with 90% identity to human ARF1. Northern blot analyses showed that levels of Arf1 mRNA are highly regulated during 12 h/12 h light/dark (LD) cycles. A biphasic pattern of expression was observed: a transient peak of Arf1 mRNA occurred at the onset of the light period, which was followed ca. 12 h later by a more prominent peak in the early to mid-dark period. When LD-synchronized cells were shifted to continuous darkness, the dark-specific peak of Arf1 mRNA persisted, indicative of a circadian rhythm. The increase in Arf1 mRNA at the beginning of the light period, however, was shown to be light-dependent, and, moreover, dependent on photosynthesis, since it was prevented by DCMU. We conclude that the biphasic pattern of Arf1 mRNA accumulation during LD cycles is due to regulation by two different factors, light (which requires photosynthesis) and the circadian clock. Thus, these studies identify a novel pattern of expression for a GTP-binding protein gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
567-77
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8534853-ADP-Ribosylation Factor 1, pubmed-meshheading:8534853-ADP-Ribosylation Factors, pubmed-meshheading:8534853-Amino Acid Sequence, pubmed-meshheading:8534853-Animals, pubmed-meshheading:8534853-Base Sequence, pubmed-meshheading:8534853-Chlamydomonas reinhardtii, pubmed-meshheading:8534853-DNA, Complementary, pubmed-meshheading:8534853-DNA Primers, pubmed-meshheading:8534853-Diuron, pubmed-meshheading:8534853-GTP-Binding Proteins, pubmed-meshheading:8534853-Gene Expression, pubmed-meshheading:8534853-Gene Expression Regulation, Plant, pubmed-meshheading:8534853-Guanosine Triphosphate, pubmed-meshheading:8534853-Humans, pubmed-meshheading:8534853-Kinetics, pubmed-meshheading:8534853-Light, pubmed-meshheading:8534853-Mammals, pubmed-meshheading:8534853-Molecular Sequence Data, pubmed-meshheading:8534853-Plants, pubmed-meshheading:8534853-Polymerase Chain Reaction, pubmed-meshheading:8534853-RNA, Messenger, pubmed-meshheading:8534853-Saccharomyces cerevisiae, pubmed-meshheading:8534853-Sequence Homology, Amino Acid, pubmed-meshheading:8534853-Signal Transduction
pubmed:year
1995
pubmed:articleTitle
Novel aspects of the regulation of a cDNA (Arf1) from Chlamydomonas with high sequence identity to animal ADP-ribosylation factor 1.
pubmed:affiliation
Department of Botany, University of Texas, Austin 78713-7640.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't