8534093

Source:http://linkedlifedata.com/resource/pubmed/id/8534093

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0022558, umls-concept:C0034510, umls-concept:C0042333, umls-concept:C0317975, umls-concept:C0871468
pubmed:issue	12
pubmed:dateCreated	1996-1-29
pubmed:abstractText	Genetic diversity among isolates of the bacterial plant pathogen Burkholderia solanacearum (synonym Pseudomonas solanacearum) race 3 biovar II of Kenya was determined by PCR with repetitive sequences (ERIC and BOX repetitive primer sets) and pulsed-field gel electrophoresis of genomic DNA digested by rare-cutting restriction endonucleases (RC-PFGE). The study comprised 46 isolates collected during 1992 from the major potato-growing regions of Kenya (45 were identified as race 3 biovar II, and 1 belonged to race 3 biovar N2) and 39 reference isolates from 19 other countries. RC-PFGE identified 10 distinct profile types among the Kenyan race 3 biovar II isolates (29 of the isolates exhibited identical profiles) and a further 27 distinct profile types among the reference isolates. ERIC and BOX primer sets were unable to differentiate race 3 biovar II isolates within the Kenyan population but differentiated a further two distinct profile types among the reference isolates. The race 3 biovar N2 isolate had a highly distinct RC-PFGE and repetitive sequence PCR profile. Statistical analysis of the data identified biogeographic trends consistent with conclusions drawn from previous studies on the origin and worldwide dissemination of race 3 biovar II isolates; however, genomic fingerprinting by RC-PFGE revealed a level of genetic diversity previously unrealized.
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-1283774, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-1350612, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-1482194, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-16348318, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-16349099, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-16349373, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-1686443, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-2425735, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-7765308, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-7952185, http://linkedlifedata.com/resource/pubmed/commentcorrection/8534093-8074510
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7605801
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Dec
pubmed:issn	0099-2240
pubmed:author	pubmed-author:HoldernessMM, pubmed-author:OffordL CLC, pubmed-author:SaddlerG SGS, pubmed-author:SmithJ JJJ
pubmed:issnType	Print
pubmed:volume	61
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	4263-8
pubmed:dateRevised	2010-9-10
pubmed:meshHeading	pubmed-meshheading:8534093-Genetic Variation, pubmed-meshheading:8534093-Kenya, pubmed-meshheading:8534093-Pseudomonas
pubmed:year	1995
pubmed:articleTitle	Genetic diversity of Burkholderia solanacearum (synonym Pseudomonas solanacearum) race 3 in Kenya.
pubmed:affiliation	International Mycological Institute, Egham, Surrey, United Kingdom.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't