Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-2-1
pubmed:abstractText
Many transcription factors are regulated by post-translational phosphorylation mechanisms. This chapter described several approaches that have been utilized to examine the phosphorylation of the c-Jun transcription factor. A combination of the techniques described in this chapter can be used to determine whether a transcription factor is phosphorylated in vivo, to analyze the sites of phosphorylation in vitro, and to permit the identification of putative protein kinases that may mediate this phosphorylation in vivo. Proteins labeled with 32P by either in vivo or in vitro techniques can be further analyzed by tryptic phosphopeptide mapping or phosphoamino acid analysis. These procedures have been described in detail elsewhere.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
254
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
550-64
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8531714-Adenosine Triphosphate, pubmed-meshheading:8531714-Animals, pubmed-meshheading:8531714-Autoradiography, pubmed-meshheading:8531714-Cells, Cultured, pubmed-meshheading:8531714-Cloning, Molecular, pubmed-meshheading:8531714-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:8531714-Glutathione Transferase, pubmed-meshheading:8531714-Indicators and Reagents, pubmed-meshheading:8531714-Phosphates, pubmed-meshheading:8531714-Phosphorus Radioisotopes, pubmed-meshheading:8531714-Phosphorylation, pubmed-meshheading:8531714-Protein Biosynthesis, pubmed-meshheading:8531714-Protein Processing, Post-Translational, pubmed-meshheading:8531714-Proto-Oncogene Proteins c-jun, pubmed-meshheading:8531714-Rabbits, pubmed-meshheading:8531714-Recombinant Fusion Proteins, pubmed-meshheading:8531714-Reticulocytes, pubmed-meshheading:8531714-Transcription Factors
pubmed:year
1995
pubmed:articleTitle
Phosphorylation of transcription factors.
pubmed:affiliation
Department of Medicine, University of Alabama at Birmingham, 35294, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't