Linked Life Data

8531019

Source:http://linkedlifedata.com/resource/pubmed/id/8531019

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-1-31
pubmed:abstractText
Individual exoantigens from 13 Paracoccidioides brasiliensis isolates were obtained and tested against a panel of 50 sera from patients with paracoccidioidomycosis by immunodiffusion test. The index of positivity varied from 20 to 100% according to the isolate. When these exoantigens were analysed by SDS-PAGE, eight presented high amounts of the glycoprotein gp43, two presented small amounts and in three there was no detectable gp43. The eight isolates presenting high amounts of gp43 were submitted to isoelectric focusing, blotted to nitrocellulose membranes, and revealed by monoclonal and polyclonal anti-gp43 antibodies. Four gp43 isoform profiles were obtained: profile A presenting pIs of 6.0, 6.2, 6.6 and 7.0, profile B presenting pIs of 6.4, 6.8 and 7.2, profile C presenting pI > 8.5 and profile D, presenting pIs of 5.8, 6.2 and 6.6. In each pattern observed, a major band with a distinct pI was detected. Despite this variation, our results strongly suggest an epitopic conservation among all isoforms analysed, since all of them were recognized by anti-gp43 monoclonal antibodies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0268-1218
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
223-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Expression and isoforms of gp43 in different strains of Paracoccidioides brasiliensis.
pubmed:affiliation
Universidade Federal de São Paulo, Escola Paulista de Medicina, Disciplina de Biologia Celular, São Paulo, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't