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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1977-6-11
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pubmed:abstractText |
The adherence, phagocytic activity and buoyant density of mouse peritoneal exudate colony forming units (CFU-PE) were investigated. There was a significant enrichment in the proportion of CFU-PE in the adherent cells population, defined as cells adhering to a plastic surface within 30 minutes of incubation. The phagocytic activity of CFU-PE was studied by incubating exudate cells with iron particles for 45 minutes. The cells were then separated into phagocytic and non-phagocytic cell fractions by passing the incubation mixture through a magnetic field. A significant enrichment of CFU-PE was seen in the phagocytic cell fraction. When exudate cells were fractionated in a Ficoll discontinuous density gradient, more than 88% of CFU-PE were recovered at the 16/18% and 18/20% interfaces. It is concluded that CFU-PE are adherent cells, have strong phagocytic activity and have a buouant density between 1.0562 and 1.0703. When bone marrow cells were studied by these techniques, the committed stem cells for both granulocytes and marcophages (CFU-C) were enriched in both non-adherent cell and non-phagocytic cells populations. In the Ficoll density gradient, CFU-C banded at a heavier density region than CFU-PE.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9541
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
407-13
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:853062-Animals,
pubmed-meshheading:853062-Ascitic Fluid,
pubmed-meshheading:853062-Bone Marrow,
pubmed-meshheading:853062-Bone Marrow Cells,
pubmed-meshheading:853062-Cell Adhesion,
pubmed-meshheading:853062-Cell Division,
pubmed-meshheading:853062-Centrifugation, Density Gradient,
pubmed-meshheading:853062-Female,
pubmed-meshheading:853062-Femur,
pubmed-meshheading:853062-Lymphocytes,
pubmed-meshheading:853062-Macrophages,
pubmed-meshheading:853062-Male,
pubmed-meshheading:853062-Mice,
pubmed-meshheading:853062-Mice, Inbred C3H,
pubmed-meshheading:853062-Phagocytosis
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pubmed:year |
1977
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pubmed:articleTitle |
Peritoneal exudate cells. IV. Characterization of colony forming cells.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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