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rdf:type |
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lifeskim:mentions |
umls-concept:C0010711,
umls-concept:C0033640,
umls-concept:C0178539,
umls-concept:C0205263,
umls-concept:C0851285,
umls-concept:C0871261,
umls-concept:C1417008,
umls-concept:C1704259,
umls-concept:C1704632,
umls-concept:C1704928,
umls-concept:C1705987,
umls-concept:C1706817,
umls-concept:C1879547,
umls-concept:C2911692
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pubmed:issue |
51
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pubmed:dateCreated |
1996-1-30
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pubmed:abstractText |
Previous work has shown that treatment of cells with the antimetabolite 1-beta-D-arabinofuranosylcytosine (ara-C) is associated with induction of the c-jun gene. The present studies demonstrate that ara-C activates the c-Abl non-receptor tyrosine kinase. We also demonstrate that activity of the stress-activated protein kinase (SAP kinase/JNK) is increased in ara-C-treated cells. Using cells deficient in c-Abl (Abl-/-) and after introduction of the c-abl gene, we show that ara-C-induced c-Abl activity is necessary for the stimulation of SAP kinase. Other studies using cells transfected with a SEK1 dominant negative demonstrate that ara-C-induced SAP kinase activity is SEK1-dependent. Furthermore, we show that overexpression of truncated c-Abl results in activation of the SEK1/SAP kinase cascade.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30278-81
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8530447-3T3 Cells,
pubmed-meshheading:8530447-Amino Acid Sequence,
pubmed-meshheading:8530447-Animals,
pubmed-meshheading:8530447-Cytarabine,
pubmed-meshheading:8530447-Enzyme Activation,
pubmed-meshheading:8530447-Kinetics,
pubmed-meshheading:8530447-MAP Kinase Kinase 4,
pubmed-meshheading:8530447-Mice,
pubmed-meshheading:8530447-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:8530447-Molecular Sequence Data,
pubmed-meshheading:8530447-Oligopeptides,
pubmed-meshheading:8530447-Protein Kinases,
pubmed-meshheading:8530447-Protein-Tyrosine Kinases,
pubmed-meshheading:8530447-Proto-Oncogene Proteins c-abl,
pubmed-meshheading:8530447-Recombinant Proteins,
pubmed-meshheading:8530447-Signal Transduction,
pubmed-meshheading:8530447-Substrate Specificity,
pubmed-meshheading:8530447-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
c-Abl activation regulates induction of the SEK1/stress-activated protein kinase pathway in the cellular response to 1-beta-D-arabinofuranosylcytosine.
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pubmed:affiliation |
Division of Cancer Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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