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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
50
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pubmed:dateCreated |
1996-1-26
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pubmed:databankReference |
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pubmed:abstractText |
We found a novel protein in the postmitochondria supernatant fraction of rat liver, which is soluble in 5% perchloric acid and strongly inhibits protein synthesis in a rabbit reticulocyte lysate system. The protein extracted from the supernatant fraction with 5% perchloric acid was purified by ammonium sulfate fractionation and CM-Sephadex chromatography. The protein was shown to consist of two identical subunits with a molecular mass of 14 kDa. By immunoscreening with the rabbit antisera against the protein, a cDNA encoding the protein was cloned and sequenced. The cDNA contained an open reading frame of 411 base pairs encoding a 136-amino acid protein with a molecular mass of 14,149 Da. The deduced amino acid sequence was completely identical with that constructed from all of the above peptides. Interestingly, the perchloric acid-soluble protein inhibited cell-free protein synthesis in the rabbit reticulocyte lysate system in a different manner from RNase A. The protein is likely to inhibit an initiation stage of cell-free protein synthesis. Among the rat tissues tested, the protein was located only in liver and kidney. These findings are the first report on a new inhibitor that may be involved in the regulation of protein synthesis in those tissues.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30060-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8530410-Amino Acid Sequence,
pubmed-meshheading:8530410-Animals,
pubmed-meshheading:8530410-Base Sequence,
pubmed-meshheading:8530410-Blotting, Northern,
pubmed-meshheading:8530410-Cytosol,
pubmed-meshheading:8530410-Heat-Shock Proteins,
pubmed-meshheading:8530410-Liver,
pubmed-meshheading:8530410-Male,
pubmed-meshheading:8530410-Mass Spectrometry,
pubmed-meshheading:8530410-Molecular Sequence Data,
pubmed-meshheading:8530410-Open Reading Frames,
pubmed-meshheading:8530410-Peptide Fragments,
pubmed-meshheading:8530410-Perchloric Acid,
pubmed-meshheading:8530410-Protein Biosynthesis,
pubmed-meshheading:8530410-Protein Synthesis Inhibitors,
pubmed-meshheading:8530410-Proteins,
pubmed-meshheading:8530410-Rabbits,
pubmed-meshheading:8530410-Rats,
pubmed-meshheading:8530410-Rats, Wistar,
pubmed-meshheading:8530410-Reticulocytes,
pubmed-meshheading:8530410-Ribonucleases,
pubmed-meshheading:8530410-Sequence Homology, Amino Acid,
pubmed-meshheading:8530410-Solubility
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pubmed:year |
1995
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pubmed:articleTitle |
Isolation and characterization of a novel perchloric acid-soluble protein inhibiting cell-free protein synthesis.
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pubmed:affiliation |
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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