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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1996-1-29
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pubmed:abstractText |
The BCL-2 gene product is involved in preventing apoptosis. The t(14,18) chromosomal translocation, which results in a fusion messenger RNA containing the entire coding region of BCL-2 and a portion of the immunoglobulin heavy chain gene, is commonly found in follicular lymphoma and appears to play a role in lymphomagenesis by inhibiting cell death. We tested the hypothesis that downregulation of BCL-2 would decrease accumulation of follicular lymphoma cells by treating the t(14,18)-carrying follicular lymphoma cell line WSU-FSCCL in vitro with antisense oligodeoxyribonucleotides (ODNs) directed against BCL-2. We found dose-dependent, sequence-specific inhibition of cell accumulation by an antisense unmodified ODN directed at codons 2 to 7, which downregulated BCL-2 protein levels. This effect was near maximal at an ODN concentration of 40 micrograms/mL (6.9 mumol/L), with minimal toxicity by control sense, reverse, and mutated antisense ODN at the same concentration. The pre-B leukemia cell line REH showed no sequence-specific growth inhibition by the antisense ODN at these concentrations, and BCL-2 protein levels were not altered. These data suggest that WSU-FSCCL may be useful in a murine model to optimize antisense ODN for potential therapeutic utility.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0929-1903
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
207-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8528964-Base Sequence,
pubmed-meshheading:8528964-Cell Division,
pubmed-meshheading:8528964-Dose-Response Relationship, Drug,
pubmed-meshheading:8528964-Down-Regulation,
pubmed-meshheading:8528964-Flow Cytometry,
pubmed-meshheading:8528964-Humans,
pubmed-meshheading:8528964-Immunohistochemistry,
pubmed-meshheading:8528964-Lymphoma, Non-Hodgkin,
pubmed-meshheading:8528964-Molecular Sequence Data,
pubmed-meshheading:8528964-Oligonucleotides, Antisense,
pubmed-meshheading:8528964-Proto-Oncogene Proteins,
pubmed-meshheading:8528964-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:8528964-Tumor Cells, Cultured
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pubmed:year |
1995
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pubmed:articleTitle |
Antisense oligodeoxyribonucleotide down-regulation of bcl-2 gene expression inhibits growth of the low-grade non-Hodgkin's lymphoma cell line WSU-FSCCL.
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pubmed:affiliation |
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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