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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1996-1-19
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pubmed:abstractText |
Studies were conducted to determine whether 1) glass fibers can induce morphological transformation in BALB/c-3T3 cells, 2) the transforming activity of glass fibers is related to fiber size, and 3) transformed cells induced by glass fibers possess neoplastic properties. In the transformation assay, BALB/c-3T3 cells were treated with three different types of glass fibers: Manville code 100 (JM-100, Manville Corp., Denver, CO), Owens-Corning AAA-10 (AAA-10, Owens-Corning Corp., Toledo, OH), and Owens-Corning general building insulation (ISL, Owens-Corning Corp.) fibers. The neoplastic properties were investigated using the soft agar cloning and gene transfection methods. All three different glass fibers were cytotoxic at high concentrations and induced dose-related increases in morphological transformation. The transforming activity was inversely related to fiber size, with AAA-10 showing higher activity than JM-100 and JM-100 showing higher activity than ISL fiber. Transformed cells induced by glass fibers exerted anchorage-independent growth (90%) and DNA transfection-mediated transformation (100%). These results indicate that glass fibers are capable of transforming mammalian (BALB/c-3T3) cells in vitro as a function of their physical properties and that glass fiber-induced transformed cells possess preneoplastic characteristics.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0270-3211
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
63-71
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8525469-3T3 Cells,
pubmed-meshheading:8525469-Animals,
pubmed-meshheading:8525469-Carcinogens,
pubmed-meshheading:8525469-Cell Adhesion,
pubmed-meshheading:8525469-Cell Transformation, Neoplastic,
pubmed-meshheading:8525469-Dose-Response Relationship, Drug,
pubmed-meshheading:8525469-Glass,
pubmed-meshheading:8525469-Mice,
pubmed-meshheading:8525469-Mice, Inbred BALB C,
pubmed-meshheading:8525469-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
Morphological transformation induced by glass fibers in BALB/c-3T3 cells.
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pubmed:affiliation |
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505-2888, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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