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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5243
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pubmed:dateCreated |
1996-1-22
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pubmed:abstractText |
Mutations in the ETR1 gene of Arabidopsis thaliana confer insensitivity to ethylene, which indicates a role for the gene product in ethylene signal transduction. Saturable binding sites for [14C]ethylene were detected in transgenic yeast expressing the ETR1 protein, whereas control yeast lacking ETR1 showed no detectable ethylene binding. Yeast expressing a mutant form of ETR1 (etr1-1) also showed no detectable ethylene binding, which provides an explanation for the ethylene-insensitive phenotype observed in plants carrying this mutation. Expression of truncated forms of ETR1 in yeast provided evidence that the amino-terminal hydrophobic domain of the protein is the site of ethylene binding. It was concluded from these results that ETR1 acts as an ethylene receptor in Arabidopsis.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1809-11
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:8525372-Arabidopsis,
pubmed-meshheading:8525372-Binding Sites,
pubmed-meshheading:8525372-Cloning, Molecular,
pubmed-meshheading:8525372-Ethylenes,
pubmed-meshheading:8525372-Genes, Plant,
pubmed-meshheading:8525372-Mutagenesis, Site-Directed,
pubmed-meshheading:8525372-Peptide Fragments,
pubmed-meshheading:8525372-Plant Proteins,
pubmed-meshheading:8525372-Receptors, Cell Surface,
pubmed-meshheading:8525372-Recombinant Proteins,
pubmed-meshheading:8525372-Saccharomyces cerevisiae
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pubmed:year |
1995
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pubmed:articleTitle |
Ethylene-binding sites generated in yeast expressing the Arabidopsis ETR1 gene.
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pubmed:affiliation |
Department of Botany, University of Wisconsin, Madison 53706, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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