Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
1996-1-24
|
pubmed:databankReference | |
pubmed:abstractText |
Cloning and characterization of the gor gene of the lactic acid bacterium Streptococcus thermophilus, encoding glutathione reductase, are described in this paper. This enzyme is a part of the enzymatic defences against oxidative stress in eukaryotic cells and in Gram-negative bacteria, but was never found in Gram-positive bacteria before this study. The amino acid sequence shares extensive similarities with glutathione reductases from other organisms, e.g. 62% amino acid identity with Escherichia coli protein. Northern blot analysis and glutathione reductase enzyme assays gave evidence that the gene is expressed in aerobically growing cells.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0923-2508
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
146
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
371-83
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:8525054-Base Sequence,
pubmed-meshheading:8525054-Blotting, Northern,
pubmed-meshheading:8525054-Cloning, Molecular,
pubmed-meshheading:8525054-DNA, Bacterial,
pubmed-meshheading:8525054-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8525054-Genes, Bacterial,
pubmed-meshheading:8525054-Glutathione Reductase,
pubmed-meshheading:8525054-Molecular Sequence Data,
pubmed-meshheading:8525054-Polymerase Chain Reaction,
pubmed-meshheading:8525054-Restriction Mapping,
pubmed-meshheading:8525054-Streptococcus
|
pubmed:year |
1995
|
pubmed:articleTitle |
Characterization of the gor gene of the lactic acid bacterium Streptococcus thermophilus CNRZ368.
|
pubmed:affiliation |
Laboratoire de Génétique et Microbiologie, UA INRA 952, Université Henry Poincaré Nancy 1, Faculté des Sciences, Vandoeuvre-lès-Nancy, France.
|
pubmed:publicationType |
Journal Article,
In Vitro
|