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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1996-1-25
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pubmed:abstractText |
An approach was sought to increase the half-life and target cell specificity of antisense oligodeoxynucleotides (oligos). A monoclonal antibody (MAb) was derived from mice immunised with an oligo complementary to a region (1-20) of the HIV genome. This MAb exerts a protective effect on the oligo from the degradation induced by plasma exonucleases in vitro and in vivo. Moreover the anti-oligo MAb dissociates from the oligo in the presence of its complementary sequence to allow hybridization of the two complementary strands. To direct the oligo to CD4+ cells the anti-oligo MAb was cross-linked to an anti-CD4 MAb. The heteroaggregate determines a 5-fold increase in the cellular membrane binding of the oligo to CD4+ lymphocytes. These findings suggest a new approach to enhancing the therapeutic action and the target specificity of antisense oligodeoxynucleotides useful for the selective inhibition of HIV replication in vivo.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-1371582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-1841656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-1960414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-2004450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-2424026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-2458490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-3036552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-3041414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-3174622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-3174646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-3264319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-6337705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-7511550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-8351515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8524649-8494718
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4603-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8524649-Animals,
pubmed-meshheading:8524649-Antibodies, Monoclonal,
pubmed-meshheading:8524649-Antigens, CD4,
pubmed-meshheading:8524649-Antiviral Agents,
pubmed-meshheading:8524649-Base Sequence,
pubmed-meshheading:8524649-CD4-Positive T-Lymphocytes,
pubmed-meshheading:8524649-Cell Line,
pubmed-meshheading:8524649-Exodeoxyribonucleases,
pubmed-meshheading:8524649-Female,
pubmed-meshheading:8524649-Genome, Viral,
pubmed-meshheading:8524649-HIV,
pubmed-meshheading:8524649-Half-Life,
pubmed-meshheading:8524649-Humans,
pubmed-meshheading:8524649-Mice,
pubmed-meshheading:8524649-Mice, Inbred BALB C,
pubmed-meshheading:8524649-Mice, Inbred Strains,
pubmed-meshheading:8524649-Molecular Sequence Data,
pubmed-meshheading:8524649-Oligonucleotides, Antisense
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pubmed:year |
1995
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pubmed:articleTitle |
A monoclonal antibody extends the half-life of an anti-HIV oligodeoxynucleotide and targets it to CD4+ cells.
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pubmed:affiliation |
Division of Experimental Oncology E, Istituto Nazionale Tumori, Milan, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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