|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1996-1-25
|
|
pubmed:abstractText |
Adeno-associated virus is an integrating DNA parvovirus with the potential to be an important vehicle for somatic gene therapy. A potential barrier, however, is the low transduction efficiencies of recombinant adeno-associated virus (rAAV) vectors. We show in this report that adenovirus dramatically enhances rAAV transduction in vitro in a way that is dependent on expression of early region 1 and 4 (E1 and E4, respectively) genes and directly proportional to the appearance of double-stranded replicative forms of the rAAV genome. Expression of the open reading frame 6 protein from E4 in the absence of E1 accomplished a similar but attenuated effect. The helper activity of adenovirus E1 and E4 for rAAV gene transfer was similarly demonstrated in vivo by using murine models of liver- and lung-directed gene therapy. Our data indicate that conversion of a single-stranded rAAV genome to a duplex intermediate limits transduction and usefulness for gene therapy.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-1206799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-1657596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-1731342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2156265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2536986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2547998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2820138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2824848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2829430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2834728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2835153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2835501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2847025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2911117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2946406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-2986797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-3012864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-3018511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-3029431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-3041032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-3485200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-3864995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-4032537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-4291934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-4627331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-5217441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6086948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6086952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6088786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6093102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6142122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6251278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6277513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6292524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6305001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6310575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6321111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-6699935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-7679117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-7842013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-7966621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-7981305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-8353427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-8460485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8523565-8474183
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
70
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
520-32
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8523565-Adenovirus E1 Proteins,
pubmed-meshheading:8523565-Adenovirus E4 Proteins,
pubmed-meshheading:8523565-Animals,
pubmed-meshheading:8523565-Cell Line,
pubmed-meshheading:8523565-Cercopithecus aethiops,
pubmed-meshheading:8523565-DNA, Single-Stranded,
pubmed-meshheading:8523565-DNA, Viral,
pubmed-meshheading:8523565-Dependovirus,
pubmed-meshheading:8523565-Gene Therapy,
pubmed-meshheading:8523565-HeLa Cells,
pubmed-meshheading:8523565-Humans,
pubmed-meshheading:8523565-Lac Operon,
pubmed-meshheading:8523565-Liver,
pubmed-meshheading:8523565-Lung,
pubmed-meshheading:8523565-Mice,
pubmed-meshheading:8523565-Mice, Inbred BALB C,
pubmed-meshheading:8523565-Open Reading Frames,
pubmed-meshheading:8523565-Recombination, Genetic,
pubmed-meshheading:8523565-Transformation, Genetic,
pubmed-meshheading:8523565-Vero Cells
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Transduction with recombinant adeno-associated virus for gene therapy is limited by leading-strand synthesis.
|
|
pubmed:affiliation |
Institute for Human Gene Therapy, University of Pennsylvania Health System, Philadelphia, USA.
|
