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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-1-24
|
| pubmed:abstractText |
Aromatic L-amino acid decarboxylase (AADC) is found in both neuronal cells and nonneuronal cells, and a single gene encodes rat AADC in both neuronal and nonneuronal tissues. However, two cDNAs for this enzyme have been identified: one from the liver and the other from pheochromocytoma. Exons 1a and 1b are found in the liver cDNA and the pheochromocytoma cDNA, respectively. In the third exon (exon 2), there are two alternatively utilized splicing acceptors specific to these exons, 1a and 1b. Structural analysis of the rat AADC gene showed that both alternative promoter usage and alternative splicing are operative for the differential expression of this gene. To demonstrate whether alternative promoter usage and splicing are tissue specific and whether the exons 1a and 1b are differentially and specifically transcribed in nonneuronal and neuronal cells, respectively, in situ hybridization histochemistry for the rat brain, adrenal gland, liver, and kidney was carried out using these two exon probes. The exon 1a probe specifically identified AADC mRNA only in nonneuronal cells, including the liver and kidney, and the exon 1b probe localized AADC mRNA to monoaminergic neurons in the CNS and the adrenal medulla. Thus, both alternative promoter usage and differential splicing are in fact operative for the tissue-specific expression of the rat AADC gene.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
66
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
14-9
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8522946-Adrenal Gland Neoplasms,
pubmed-meshheading:8522946-Adrenal Medulla,
pubmed-meshheading:8522946-Animals,
pubmed-meshheading:8522946-Aromatic-L-Amino-Acid Decarboxylases,
pubmed-meshheading:8522946-Base Sequence,
pubmed-meshheading:8522946-Brain,
pubmed-meshheading:8522946-DNA, Complementary,
pubmed-meshheading:8522946-Enzyme Induction,
pubmed-meshheading:8522946-Exons,
pubmed-meshheading:8522946-In Situ Hybridization,
pubmed-meshheading:8522946-Kidney,
pubmed-meshheading:8522946-Liver,
pubmed-meshheading:8522946-Male,
pubmed-meshheading:8522946-Molecular Sequence Data,
pubmed-meshheading:8522946-Nerve Tissue Proteins,
pubmed-meshheading:8522946-Neurons,
pubmed-meshheading:8522946-Organ Specificity,
pubmed-meshheading:8522946-Pheochromocytoma,
pubmed-meshheading:8522946-Promoter Regions, Genetic,
pubmed-meshheading:8522946-RNA, Messenger,
pubmed-meshheading:8522946-RNA Splicing,
pubmed-meshheading:8522946-Rats,
pubmed-meshheading:8522946-Rats, Sprague-Dawley
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Alternate promoters in the rat aromatic L-amino acid decarboxylase gene for neuronal and nonneuronal expression: an in situ hybridization study.
|
| pubmed:affiliation |
Laboratory of Molecular Neurobiology, Cornell University Medical College, W. M. Burke Medical Research Institute, White Plains, New York 10605, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|