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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-1-25
|
| pubmed:databankReference | |
| pubmed:abstractText |
HLA-A30 is present in the Sardinian population at a frequency of 23%. We have designed a system using nested ARMS-PCR to determine the relative frequencies of the HLA-A*30 allelic variants (A*3001, A*3002, and A*3003) within this population. The use of a nested PCR approach, in which the first-round reaction provides HLA-A*30 specificity and template DNA for the subsequent nested reactions, is a powerful means of discriminating between alleles of very similar sequence. Using this method, we performed subtyping of 35 serologically defined HLA-A30 Sardinian individuals, and taking into account homozygotes, identified 38 A*30 alleles. Of these, 33 typed as A*3002, four typed as A*3001, and one sample did not conform to the patterns of reactivity of any of the published A*30 alleles. Haplotype information showed strong linkage disequilibrium between A*3002 and B18. This study underlines the potential of DNA-based methods for typing HLA class I in terms of adding further levels of definition to studies of population structure and also as a means of identifying new alleles.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0198-8859
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
35-42
|
| pubmed:dateRevised |
2011-5-16
|
| pubmed:meshHeading |
pubmed-meshheading:8522453-Alleles,
pubmed-meshheading:8522453-Amino Acid Sequence,
pubmed-meshheading:8522453-Base Sequence,
pubmed-meshheading:8522453-Consensus Sequence,
pubmed-meshheading:8522453-DNA Mutational Analysis,
pubmed-meshheading:8522453-Gene Frequency,
pubmed-meshheading:8522453-Genetic Variation,
pubmed-meshheading:8522453-Genotype,
pubmed-meshheading:8522453-HLA-A Antigens,
pubmed-meshheading:8522453-Histocompatibility Testing,
pubmed-meshheading:8522453-Humans,
pubmed-meshheading:8522453-Isoelectric Focusing,
pubmed-meshheading:8522453-Italy,
pubmed-meshheading:8522453-Linkage Disequilibrium,
pubmed-meshheading:8522453-Molecular Sequence Data,
pubmed-meshheading:8522453-Polymerase Chain Reaction,
pubmed-meshheading:8522453-Sequence Alignment,
pubmed-meshheading:8522453-Sequence Homology, Amino Acid
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Defining the allelic variants of HLA-A30 in the Sardinian population using amplification refractory mutation system--polymerase chain reaction.
|
| pubmed:affiliation |
ICRF, Institute of Molecular Medicine, Oxford, UK.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|