Linked Life Data

8521737

Source:http://linkedlifedata.com/resource/pubmed/id/8521737

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1996-1-19
pubmed:abstractText
The uptake and intracellular activity of fleroxacin in murine J774.1 macrophages and human polymorphonuclear leukocytes were studied. The uptake of fleroxacin by J774.1 macrophages was rapid and reversible. The cellular to extracellular concentration ratios of fleroxacin in both types of phagocytes ranged from 5 to 6. These ratios were almost equal to those of ofloxacin and ciprofloxacin, and higher than those of the beta-lactam antibiotics, flomoxef and piperacillin. The intracellular activity of fleroxacin in J774.1 macrophages, examined with Staphylococcus aurenus as a test bacterium, showed that its bactericidal action was dependent on both the extracellular concentration and the exposure time. Fleroxacin reduced the number of viable cells of ingested S. aureus at an extracellular concentration that simulated the clinical serum levels, that is, killing more than 70% of the bacteria at 4 micrograms/ml. The bactericidal activity of fleroxacin in phagocytes was superior to that of erythromycin, flomoxef and piperacillin. These results indicate that fleroxacin is taken up well by phagocytes, reaching a concentration several fold higher than the extracellular concentration, and that it has potent activity against intracellular pathogens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0009-3157
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
353-9
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:articleTitle
Uptake and intracellular activity of fleroxacin in phagocytic cells.
pubmed:affiliation
Central Research Laboratories, Kyorin Pharmaceutical Co. Ltd., Tochigi, Japan.
pubmed:publicationType
Journal Article