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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-1-24
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pubmed:abstractText |
Prostaglandin endoperoxide synthase 2, also referred to as cyclooxygenase 2 (COX-2), is a key enzyme in the conversion of arachidonic acid to prostaglandins and other eicosanoids. Rat intestinal epithelial (RIE) cells were permanently transfected with a COX-2 expression vector oriented in the sense (RIE-S) or antisense (RIE-AS) direction. The RIE-S cells expressed elevated COX-2 protein levels and demonstrated increased adhesion to extracellular matrix (ECM) proteins. E-cadherin was undetectable in RIE-S cells, but was elevated in parental RIE (RIE-P) and RIE-AS cells. RIE-S cells were resistant to butyrate-induced apoptosis, had elevated BCL2 protein expression, and reduced transforming growth factor beta 2 receptor levels. The phenotypic changes involving both increased adhesion to ECM and inhibition of apoptosis were reversed by sulindac sulfide (a COX inhibitor). These studies demonstrate that overexpression of COX-2 leads to phenotypic changes in intestinal epithelial cells that could enhance their tumorigenic potential.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
493-501
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8521479-Alkaline Phosphatase,
pubmed-meshheading:8521479-Animals,
pubmed-meshheading:8521479-Apoptosis,
pubmed-meshheading:8521479-Cadherins,
pubmed-meshheading:8521479-Cell Adhesion,
pubmed-meshheading:8521479-Epithelium,
pubmed-meshheading:8521479-Extracellular Matrix,
pubmed-meshheading:8521479-Gene Expression,
pubmed-meshheading:8521479-Intestines,
pubmed-meshheading:8521479-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:8521479-Protein Binding,
pubmed-meshheading:8521479-Protein-Tyrosine Kinases,
pubmed-meshheading:8521479-Proto-Oncogene Proteins,
pubmed-meshheading:8521479-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:8521479-Rats,
pubmed-meshheading:8521479-Receptors, Transforming Growth Factor beta
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pubmed:year |
1995
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pubmed:articleTitle |
Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase 2.
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pubmed:affiliation |
Department of Medicine, Vanderbilt University Medical Center, Veterans Affairs Medical Center, Nashville, Tennessee 37232, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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