Linked Life Data

8520687

Source:http://linkedlifedata.com/resource/pubmed/id/8520687

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1996-1-25
pubmed:abstractText
HPCE has been found to be efficient for the optimization of conjugation procedures employed for coupling of the haptens p-nitrophenyl-alpha-D-galactopyranoside (PNPG) and soyasaponin I to a carrier protein, Kunitz soybean trypsin inhibitor (KSTI). The carbohydrate moieties of the haptens were oxidized with periodate followed by reaction with epsilon-amino groups of the carrier. For PNPG, the periodate oxidations (0.01-0.2 M NaIO4) were followed by HPCE and 0.1 M periodate was chosen as the optimum concentration. The coupling of PNPG to KSTI was found to proceed at a constant rate for more than 250 min. The reaction rate for the soyasaponin conjugation to KSTI declined after 80 min of incubation. The coupling of soyasaponin to KSTI was confirmed by enzyme-linked immunosorbent assay with monoclonal antibodies directed against soyasaponin I. The method was found to be particularly powerful for the investigation of conjugates with low epitope densities that are difficult to determine otherwise.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
717
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
75-81
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Optimization of hapten-protein conjugation by high-performance capillary electrophoresis.
pubmed:affiliation
Department of Biochemistry and Nutrition, Technical University of Denmark, Lyngby, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't