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rdf:type |
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lifeskim:mentions |
umls-concept:C0008109,
umls-concept:C0040648,
umls-concept:C0040715,
umls-concept:C0553580,
umls-concept:C0599718,
umls-concept:C0599813,
umls-concept:C0599893,
umls-concept:C0808900,
umls-concept:C1510411,
umls-concept:C1514562,
umls-concept:C1522702,
umls-concept:C1546857,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2700640
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pubmed:issue |
12
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pubmed:dateCreated |
1993-7-22
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pubmed:abstractText |
The 11;22 chromosomal translocation specifically linked to Ewing sarcoma and primitive neuroectodermal tumor results in a chimeric molecule fusing the amino-terminal-encoding portion of the EWS gene to the carboxyl-terminal DNA-binding domain encoded by the FLI1 gene. We have isolated a fourth EWS-FLI1 fusion cDNA that is structurally distinct from the three forms previously described. To determine the transforming activity of this gene, alternative forms of the EWS-FLI1 fusion were transduced into NIH 3T3 cells. Cells expressing either type 1 or type 4 fusion constructs formed foci in culture and colonies in soft agar, indicating that EWS-FLI1 is a transforming gene. EWS-FLI1 deletion mutants were created to map functionally the critical regions within the chimera. Deletion of either the EWS domain or the FLI1 corresponding to the DNA-binding domain totally abrogated the ability for EWS-FLI1 to transform 3T3 cells. These data indicate that the oncogenic effect of the 11;22 translocation is caused by the formation of a chimeric transcription factor. Formation of chimeric transcription factors has now been demonstrated to promote tumors of both neuroectodermal and hematopoietic origin, suggesting that this may be a common mechanism in human carcinogenesis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1283315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1311253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1311254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1522903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1525828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1592263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1633115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1652368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1652369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1672117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1967982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1967983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1988146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-1988149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2001839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2005881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2044959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2070421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2170850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2218500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2225078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2253872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2419911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2455217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2555704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2725497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2733791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-2799390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-3004699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-3163261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-3327468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8516324-518835
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
90
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pubmed:geneSymbol |
EWS,
FLI1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5752-6
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8516324-Humans,
pubmed-meshheading:8516324-Animals,
pubmed-meshheading:8516324-Sarcoma, Ewing,
pubmed-meshheading:8516324-Base Sequence,
pubmed-meshheading:8516324-Tumor Cells, Cultured,
pubmed-meshheading:8516324-Amino Acid Sequence,
pubmed-meshheading:8516324-Cell Line,
pubmed-meshheading:8516324-Friend murine leukemia virus,
pubmed-meshheading:8516324-Transformation, Genetic,
pubmed-meshheading:8516324-Molecular Sequence Data,
pubmed-meshheading:8516324-Translocation, Genetic,
pubmed-meshheading:8516324-Oligodeoxyribonucleotides,
pubmed-meshheading:8516324-DNA-Binding Proteins,
pubmed-meshheading:8516324-Restriction Mapping,
pubmed-meshheading:8516324-Transcription Factors,
pubmed-meshheading:8516324-Chromosomes, Human, Pair 22
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