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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1993-7-9
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pubmed:abstractText |
The plasmid pH5KV14 containing the right end of the Ad5 viral genome (45-100 map units) with deleted nonessential E3 region (78.5-84 map units) has been constructed. A helper-independent recombinant virus Ad5KV14 has been obtained as a result of in vivo transformation of the line 293 human cells by the pH5KV14 plasmid DNA and an EcoRI-A DNA fragment of the virus Ad5 and subsequent recombination. A nondefective recombinant virus Ad5KV17 containing the Ad5 genome with the E3 region substituted for the plasmid DNA fragment coding for the kanamycin-resistance has been constructed by a similar procedure.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0208-0613
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8510682-Adenoviridae,
pubmed-meshheading:8510682-Cells, Cultured,
pubmed-meshheading:8510682-DNA, Recombinant,
pubmed-meshheading:8510682-Genetic Vectors,
pubmed-meshheading:8510682-Genome, Viral,
pubmed-meshheading:8510682-Humans,
pubmed-meshheading:8510682-Kanamycin Resistance,
pubmed-meshheading:8510682-Plasmids,
pubmed-meshheading:8510682-Sequence Deletion
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pubmed:articleTitle |
[Preparation of a non-defective adenovirus vector based on the Ad5 genome with the E3 region replaced with plasmid DNA with a kanamycin- resistance gene].
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pubmed:publicationType |
Journal Article,
English Abstract
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