Linked Life Data

8510205

Source:http://linkedlifedata.com/resource/pubmed/id/8510205

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1993-7-12
pubmed:abstractText
We analyzed viral recombination events that occur during the preleukemic period in AKR mice. We tagged a molecular chimera between the nonleukemogenic virus Akv and the leukemogenic mink cell focus-inducing (MCF) virus MCF 247 with an amber suppressor tRNA gene, supF. We injected the supF-tagged chimeric virus that contains all of the genes of MCF 247 except the envelope gene, which in turn is derived from Akv, into newborn AKR mice to evaluate its pathogenic potential. Approximately the same percentage of animals developed leukemia with similar latent periods when injected with either the tagged or nontagged virus. DNA from tumors induced in AKR mice by the tagged chimeric virus was analyzed by Southern blotting with the supF gene as a probe. One set of tumors contained the injected supF-tagged virus. Two kinds of supF-tagged proviruses were found in a second set of tumors. One group of supF-tagged viruses had a restriction map consistent with that of the injected virus, while the other group of proviruses had restriction maps that suggested that the proviruses had acquired an MCF virus-like envelope gene by recombination with endogenous viral sequences. These results demonstrate that injected viruses recombine in vivo with endogenous viral sequences. Furthermore, the progression to leukemia was accelerated in mice that develop tumors containing proviruses with an MCF virus env gene, emphasizing the importance of the role of the MCF virus env gene product in transformation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1061100, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1326663, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1372369, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1381539, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1527855, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1658356, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1690424, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1700865, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1847454, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-1850020, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-191826, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-200928, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-203942, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2052545, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-206722, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2154592, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2154701, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-215703, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2161534, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2164592, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-222476, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2304918, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2370688, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2384920, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2536834, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2981334, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-2982233, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-3016982, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-3039169, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-4316300, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-4332983, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-4350228, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-4376054, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-4705382, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6088801, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6181896, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6224881, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6244357, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6253201, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6261142, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6267794, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6285602, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6287025, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6296436, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6312074, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6312324, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-6328017, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-7143566, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-8213, http://linkedlifedata.com/resource/pubmed/commentcorrection/8510205-90708
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:geneSymbol
env, supF
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3763-70
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
A direct demonstration of recombination between an injected virus and endogenous viral sequences, resulting in the generation of mink cell focus-inducing viruses in AKR mice.
pubmed:affiliation
Center for Virology, Children's National Medical Center, Washington, DC 20010-2970.
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