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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1993-7-15
|
| pubmed:abstractText |
In the present study, we examined time-dependent changes in activity, mRNA and immunoreactivity of the serotonin biosynthetic enzymes, tryptophan hydroxylase (TPH) and aromatic L-amino acid decarboxylase (AADC) in dorsal raphe nucleus (DRN), caudal brainstem and hypothalamus, following intracisternal injection of 5,7-dihydroxytryptamine (5,7-DHT) in neonatal rats. TPH activity in central serotonergic cell bodies and terminals was reduced to 20-30% of control levels at 1-8 weeks after neonatal, low-dose 5,7-DHT injection (24 micrograms free base). In contrast, AADC activity was either not changed or decreased to 40% of control levels, depending on the region. In situ hybridization and immunocytochemical staining indicated that 5,7-DHT caused a marked reduction in TPH and AADC message levels as well as the number of 5-HT and AADC-immunoreactive cells within the DRN as early as 1 week after 5,7-DHT. Even 15 weeks after drug administration recovery did not occur. This apparent neuronal loss was region-specific suggesting that some serotonergic neurons are more resistant to neonatal 5,7-DHT treatment than others. Taken together, these studies indicate that neonatal treatment with 5,7-DHT produces a marked and permanent (up to 15 weeks) reduction in the number of central serotonergic neurons.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5,7-Dihydroxytryptamine,
http://linkedlifedata.com/resource/pubmed/chemical/Aromatic-L-Amino-Acid Decarboxylases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Tryptophan Hydroxylase
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0006-8993
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
23
|
| pubmed:volume |
609
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
59-66
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:8508321-5,7-Dihydroxytryptamine,
pubmed-meshheading:8508321-Animals,
pubmed-meshheading:8508321-Animals, Newborn,
pubmed-meshheading:8508321-Aromatic-L-Amino-Acid Decarboxylases,
pubmed-meshheading:8508321-Brain,
pubmed-meshheading:8508321-Brain Stem,
pubmed-meshheading:8508321-Female,
pubmed-meshheading:8508321-Hypothalamus,
pubmed-meshheading:8508321-Immunohistochemistry,
pubmed-meshheading:8508321-In Situ Hybridization,
pubmed-meshheading:8508321-Nerve Endings,
pubmed-meshheading:8508321-Neurons,
pubmed-meshheading:8508321-Pregnancy,
pubmed-meshheading:8508321-RNA, Messenger,
pubmed-meshheading:8508321-Raphe Nuclei,
pubmed-meshheading:8508321-Rats,
pubmed-meshheading:8508321-Rats, Sprague-Dawley,
pubmed-meshheading:8508321-Serotonin,
pubmed-meshheading:8508321-Time Factors,
pubmed-meshheading:8508321-Tryptophan Hydroxylase
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Changes in activity and mRNA for rat tryptophan hydroxylase and aromatic L-amino acid decarboxylase of brain serotonergic cell bodies and terminals following neonatal 5,7-dihydroxytryptamine.
|
| pubmed:affiliation |
Laboratory of Molecular Neurobiology, Cornell University Medical College, Burke Medical Research Institute, White Plains, NY 10605.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|