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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1993-7-13
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pubmed:abstractText |
When transport between the rough endoplasmic reticulum (ER) and Golgi complex is blocked by Brefeldin A (BFA) treatment or ATP depletion, the Golgi apparatus and associated transport vesicles undergo a dramatic reorganization. Because recent studies suggest that coat proteins such as beta-COP play an important role in the maintenance of the Golgi complex, we have used immunocytochemistry to determine the distribution of beta-COP in pancreatic acinar cells (PAC) in which ER to Golgi transport was blocked by BFA treatment or ATP depletion. In controls, beta-COP was associated with Golgi cisternae and transport vesicles as expected. Upon BFA treatment, PAC Golgi cisternae are dismantled and replaced by clusters of remnant vesicles surrounded by typical ER transitional elements that are generally assumed to represent the exit site of vesicular carriers for ER to Golgi transport. In BFA-treated PAC, beta-COP was concentrated in large (0.5-1.0 micron) aggregates closely associated with remnant Golgi membranes. In addition to typical ER transitional elements, we detected a new type of transitional element that consists of specialized regions of rough ER (RER) with ribosome-free ends that touched or extended into the beta-COP containing aggregates. In ATP-depleted PAC, beta-COP was not detected on Golgi membranes but was concentrated in similar large aggregates found on the cis side of the Golgi stacks. The data indicate that upon arrest of ER to Golgi transport by either BFA treatment or energy depletion, beta-COP dissociates from PAC Golgi membranes and accumulates as large aggregates closely associated with specialized ER elements. The latter may correspond to either the site of entry or exit for vesicles recycling between the Golgi and the RER.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1096303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1425761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1425762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1425763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-14731804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1496018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1500424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1555237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1740466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1840503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1894639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1898984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1898986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1924322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1957170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1986861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-1993732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-2032551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-2105715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-2178778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-2188733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-2277061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-2426273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-2722561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-2775262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-3316245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-3536963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-3554220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-3575304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-4374474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-5699933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-6035647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-6035648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-6669049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8507897-7033246
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1059-1524
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
413-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8507897-Adenosine Triphosphate,
pubmed-meshheading:8507897-Animals,
pubmed-meshheading:8507897-Brefeldin A,
pubmed-meshheading:8507897-Coatomer Protein,
pubmed-meshheading:8507897-Cyclopentanes,
pubmed-meshheading:8507897-Cytoplasm,
pubmed-meshheading:8507897-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8507897-Endoplasmic Reticulum,
pubmed-meshheading:8507897-Female,
pubmed-meshheading:8507897-Golgi Apparatus,
pubmed-meshheading:8507897-Immunohistochemistry,
pubmed-meshheading:8507897-Male,
pubmed-meshheading:8507897-Membrane Proteins,
pubmed-meshheading:8507897-Mice,
pubmed-meshheading:8507897-Microscopy, Electron,
pubmed-meshheading:8507897-Microtubule-Associated Proteins,
pubmed-meshheading:8507897-Pancreas,
pubmed-meshheading:8507897-Rats,
pubmed-meshheading:8507897-Rats, Sprague-Dawley
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pubmed:year |
1993
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pubmed:articleTitle |
Disruption of endoplasmic reticulum to Golgi transport leads to the accumulation of large aggregates containing beta-COP in pancreatic acinar cells.
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pubmed:affiliation |
Division of Cellular and Molecular Medicine, University of California, San Diego, La Jolla 92093.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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